Agrobacterium-mediated Genetic Transformation of Chickpea (Cicer arietinum L.)
Rajender Singh *
National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110 012 Directorate of Wheat Research (I.C.A.R.), Karnal 132001
Ranjeet Singh Jat
National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110 012 Directorate of Wheat Research (I.C.A.R.), Karnal 132001
Srinivasan .
National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110 012 Directorate of Wheat Research (I.C.A.R.), Karnal 132001
*Author to whom correspondence should be addressed.
Abstract
Transformation of chickpea (Cicer arietinum L.) was achieved using Agrobacterium tumefaciens strain EHA105 harboring binary vector p35SGUSINT containing uidA (GUS) and npt II genes, and pJB90GI containing uid A (GUS) and hpt genes. A strong GUS expression was detected in the putative transformed shoots by the histochemical assay. Transformation was confirmed by the presence of 0.69kb and 0.82kb amplified fragments for npt II and hpt genes, respectively, by PCR analysis. Multiple shoot formation was achieved in cotyledonary notch region on MS medium supplemented with 1.0 mM thidiazuron.
Keywords: Cicer arietinum, Agrobacterium tumefaciens, Thidiazuron, Transformation, Regeneration