Microsatellite Markers for Yellow Stem Borer (Scirpophaga incertulas Walker.) Resistance in Rice
A. Selvi *
Division of Crop Improvement, Sugarcane Breeding Institute, Coimbatore- 641007
P. Shanmugasundaram
Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641007
S. Mohankumar
Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641007
J. A. J. Raja
Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641007
S. Sadasivam
Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641007
*Author to whom correspondence should be addressed.
Abstract
The availability of molecular markers for various traits is a prerequisite for Marker Assisted Selection, Map based cloning etc. The possibility to tag genes for yellow stem borer resistance with microsatellite markers was investigated in the present study. An F2 mapping population was derived from a cross between the moderately resistant variety W1263 and a highly susceptible variety Co43. Parental survey using 48 microsatellite primer pairs revealed that 25 primers were polymorphic between the parents. Bulk Segregant Analysis (BSA) with the F2 mapping population showing extreme cases of resistance and susceptibility could identify two primers RM241 and RM219 segregating with the resistant and susceptible bulk. Further analysis with F2 individuals revealed that the marker RM241 is associated with the trait.
Keywords: Bulk segregant analysis, Microsatellite markers, Gene tagging, Yellow stem borer resistance