In Vitro Plant Regeneration from Shoot Tip Explants of Panicum sumatrence
K. Vasanth
Plant Tissue Culture Unit, Department of Plant Science, School of Life Sciences Bharathidasan University, Tiruchirappalli-620 024, India
A. Lakshmi Prabha
Plant Tissue Culture Unit, Department of Plant Science, School of Life Sciences Bharathidasan University, Tiruchirappalli-620 024, India
M. Jeyakumar
Plant Tissue Culture Unit, Department of Plant Science, School of Life Sciences Bharathidasan University, Tiruchirappalli-620 024, India
A. Muthusamy
Plant Tissue Culture Unit, Department of Plant Science, School of Life Sciences Bharathidasan University, Tiruchirappalli-620 024, India
N. Jayabalan *
Plant Tissue Culture Unit, Department of Plant Science, School of Life Sciences Bharathidasan University, Tiruchirappalli-620 024, India
*Author to whom correspondence should be addressed.
Abstract
In vitro raised shoot tip explants from little millet were used to produce multiple shoots on a medium containing various concentrations of growth regulators. Among the combinations used, MS medium containing BAP (0.3 mg/l) and KIN (0.2 mg/l) combination was found to be best for induction of multiple shoots within four weeks of culture. These shoots were transferred to shoot elongation medium containing BAP 0.2 mg/l and GA3 0.3 mg/l for further growth. The elongated shoots were separated individually and transferred to rooting media containing different concentrations of NAA and IBA. The highest percentage of rooting was observed with 0.3 mg/l) of NAA and maximum root length was recorded with IBA at 0.3 mg/l. The regenerated plantlets were transferred to the field after hardening.
Keywords: Multiple shoot induction, Plantlet regeneration, Panicum scumatrence, Shoot tip explants