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A protocol for in vitro propagation through axillary buds culture for a promising winter hardy form of Rosa canina used as a rootstock for ornamental roses was developed.
Axillary meristems isolated in September-October were successfully established for regeneration of adventitious shoots in hormone-free modified liquid Murashige and Skoog (MS) medium with 100 mg l-1 of glutathione and 3% (w/v) glucose in darkness for 2-3 days followed by subculture on induction MS medium supplemented with 2.0 mg l-1 benzyladenine (BA) and 1.0 mg l-1 indole-3-acetic acid (IAA). The highest multiplication rate (7.5 shoots per explant) was archieved on MS medium supplemented with 1.0 mg l-1 BA. Rooting was induced on half-strength MS medium supplemented with 1.0 mg l-1 IAA. The regenerated plants were acclimatized in containers with sterile sand followed by transferring into pots containing a peat with perlite, mold, sand and coconut substrate (1:1:0.5:0.5) and finally were planted in the field. The use of biotechnological techniques allowed obtaining uniformed clonal material of rootstocks for ornamental roses with the best parameters of inoculation zone, compared with the plants propagated by green cutting.