A NEW SIMPLE AND EFFICIENT DNA EXTRACTION PROTOCOL FOR ORCHID WITHOUT LIQUID NITROGEN AND PHENOL
JOYRISON KAMBA
Department of Botany, Nagaland University, Lumami 798627, Nagaland, India
CHITTA RANJAN DEB *
Department of Botany, Nagaland University, Lumami 798627, Nagaland, India
*Author to whom correspondence should be addressed.
Abstract
With the advancement of molecular biology techniques many conventional genomic DNA extraction protocols has been developed for good, pure, high quality DNA with expensive and hazardous chemical reagents. Here we present a rapid, simple and efficient DNA extraction protocol using cetyl trimethylammonium bromide (CTAB) for orchid without involvement of liquid nitrogen and toxic phenols which are environmentally hazardous. The extracted genomic DNA was successfully used for Polymerase Chain Reaction (PCR) amplification of plant barcode genes (rbcL, matK and ITS) for orchid species.
Keywords: DNA extraction, CTAB, orchid, liquid nitrogen, phenol, PCR, barcode