EFFECT OF SALICYLIC ACID ON THE BIOSYNTHESIS OF SOLASODINE IN CELL SUSPENSION CULTURE OF Solanum hainanense HANCE
NGUYEN HUU THUAN ANH
Department of Biotechnology, College of Sciences, Hue University, 77 Nguyen Hue Street, Hue 530000, Vietnam
TON NU THUY AN
Department of Biotechnology, College of Sciences, Hue University, 77 Nguyen Hue Street, Hue 530000, Vietnam
NGUYEN THUAN NHO
Department of Biotechnology, College of Sciences, Hue University, 77 Nguyen Hue Street, Hue 530000, Vietnam
VO THI VIEN DUNG
Department of Biotechnology, College of Sciences, Hue University, 77 Nguyen Hue Street, Hue 530000, Vietnam
NGUYEN HOANG LOC *
Department of Biotechnology, College of Sciences, Hue University, 77 Nguyen Hue Street, Hue 530000, Vietnam
*Author to whom correspondence should be addressed.
Abstract
In our study, the elicitation effect of salicylic acid on the growth and solasodine production of Solanum hainanense Hance cells were investigated. The results showed that the addition of various concentrations of salicylic acid (50 to 250 µM) to the medium had caused different eliciting influences. The highest production of solasodine (245.2 mg/g dry weight) induced by 150 µM salicylic acid at the beginning of cell culture was 2- and 10.8-fold in comparison with that of non-elicited cells and in planta 1-year-old root, respectively.
Keywords: Cell suspension, elicitor, salicylic acid, Solanum hainanense, solasodine