STUDIES ON IN VITRO MORPHOGENESIS FROM BULB SCALE AND LEAF DISC EXPLANT OF AMARYLLIS BELLADONNA L.
S. SARATHE
Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India
M. K. TRIPATHI *
Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India
M. VIDHYA SANKAR
Department of Floriculture and Landscaping, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India
O. P. SINGH
Department of Plant Physiology, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India
*Author to whom correspondence should be addressed.
Abstract
An efficient method for rapid propagation of Amaryllis belladonna L. has been developed in this study. Bulb scales and leaf discs were used as explants and cultured on MS medium amended with diverse auxins and cytokinins in varying concentrations as sole as well as in different combinations. For bulb scale culture, culture media MS3N.5B (MS+ 3.0 mg.l-1NAA + 0.5 mg.l-1 BA) proved well for callus initiation. Culture media MS3B(MS+ 3.0mg.l-1 BA) exhibited higher shoot proliferating efficiency, while number of shoot (s) per explant and shoot of higher length were documented on nutrient medium MSB.5N (MS+ 1.0 mg.l-1BA + 0.5 mg.l-1 NAA). For cultured leaf disc, inoculation medium MS2D.5B (MS+ 2.0mg.l-12,4-D + 0.5 mg.l-1 BA) initiated higher degree of callus induction. Elevated mrophogenic calli formation was displayed by culture medium MS2N.5B(MS+ 2.0mg.l-1NAA + 0.5 mg.l-1 BA) and plantlets in higher number (s) were regenerated on regeneration medium MS.5Td(MS+ 0.5. mg.l-1TDZ). Higher in vitro rooting responses i.e. root proliferating efficiency, number of root(s) and root of higher length was recorded on culture medium MS2IB (MS+ 2.0mg.l-1IBA).
Keywords: Amaryllis belladonna, bulb scale culture, leaf disc culture, organogenesis, embryogenesis and plantlet regeneration