Biotechnology Frontiers in 21st Century Agriculture: A Comprehensive Review

M. Ramachandra Naik

Department of Botany, BLDEA`s, S.B Arts and K.C.P Science College, Vijayapur-586103, Karnataka, India. b Sandip Institute of Pharmaceutical Sciences, Nashik, India.

Sagar Kashinath Khokale *

Sandip Institute of Pharmaceutical Sciences, Nashik, India.

B. S. Ravishankar

Department of Zoology, BLDEA's, Commerce, BHS Arts & TGP Science College, Jamkhandi Taluk, Bagalkot District, Karnataka State, India.

Sandeep `Rout

Sri Sri University, Cuttack, Odisha-754006, India.

Narinder Panotra

Institute of Biotechnology, SKUAST, Jammu and Kashmir, 180009, India.

Suvidha Mishra

Institute of Agriculture Sciences, SAGE University, Indore, India.

Lalit Upadhyay

SKUAST, Jammu and Kashmir, India.

Vimal Kumar

School of Agricultural Sciences, IIMT University, Meerut, India.

Shubham Gupta

School of Agricultural Sciences and Engine, IFTM University, India.

Akash Yadav

Bundelkhand University, Jhansi, (U.P.), India.

*Author to whom correspondence should be addressed.


Abstract

Agricultural biotechnology has emerged as a critical frontier in addressing the multifaceted challenges of 21st-century food systems, driven by a growing global population and the intensifying impacts of climate change. This review explores the transformative potential of modern biological tools, including genetic engineering, CRISPR-Cas9 genome editing, and multi-omics integration, in enhancing crop resilience and productivity. By synthesizing current research, the paper highlights how these technologies mitigate both biotic stresses, such as viral and fungal pathogens, and abiotic stresses, including drought, salinity, and extreme temperatures. Beyond yield enhancement, biotechnology plays a pivotal role in nutritional biofortification and environmental stewardship, notably through the reduction of synthetic pesticide reliance and the sequestration of carbon emissions. The analysis further discusses the transition toward a circular bio-economy, utilizing agricultural residues for bio-refining and biodegradable packaging. However, the global adoption of these innovations remains uneven, hindered by stringent regulatory frameworks, public safety concerns, and policy lags in developing regions.1 To realize the goal of a sustainable "Evergreen Revolution," the review emphasizes the necessity of harmonized biosafety guidelines and the integration of artificial intelligence with molecular breeding. Ultimately, biotechnology stands as an indispensable pillar for ensuring global food security, offering precise solutions to maintain ecological balance while meeting the demands of a projected population of nine billion by 2030.

Keywords: Agricultural biotechnology, CRISPR-Cas9, food security, climate resilience, genetic engineering, sustainable agriculture, biofortification


How to Cite

Naik, M. Ramachandra, Sagar Kashinath Khokale, B. S. Ravishankar, Sandeep `Rout, Narinder Panotra, Suvidha Mishra, Lalit Upadhyay, Vimal Kumar, Shubham Gupta, and Akash Yadav. 2026. “Biotechnology Frontiers in 21st Century Agriculture: A Comprehensive Review”. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY 27 (3-4):124-43. https://doi.org/10.56557/pcbmb/2026/v27i3-410371.

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