PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Molecular markers have become indispensable tools in crop improvement as they enable precise genotype-based selection of desirable traits. Advances in next-generation sequencing (NGS) have facilitated the identification and development of a wide range of DNA markers, including simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). Also approaches such as genotyping-by-sequencing (GBS) and restriction-site associated DNA sequencing (RAD-seq) provide more cost-effective strategies for generating genome-wide marker datasets. Cytogenetic and epigenetic markers have further expanded traditional marker systems by offering insights into chromosomal structural variation, gene regulation, and epigenetic inheritance. Integration of molecular markers with multi-omics approaches enables a comprehensive understanding of complex traits and accelerates breeding programs through the identification of key genes and regulatory mechanisms. Molecular markers are widely used in crop improvement including genetic diversity analysis and, in the identification, selection, and transfer of desirable traits. However, their effective utilization remains constrained by high costs, infrastructure and skill requirements, data management challenges, and biological complexities such as polygenic traits, limited polymorphism, and polyploid genomes.