Journal of Basic and Applied Research International

Airborne fungal contamination poses significant public health risks in healthcare settings, particularly in primary health centres serving rural and semi-urban populations. The present study investigated the seasonal distribution, diversity, and statistical variation of aeromycoflora at the Primary Health Centre (PHC), Baghera, Durg, Chhattisgarh, over a one-year period (March 2022–February 2023). Air samples from indoor and outdoor environments were collected using the settle plate technique and analyzed season-wise. A total of 54 fungal taxa indoors and 48 taxa outdoors, belonging to Zygomycotina, Ascomycotina, Basidiomycota, and anamorphic fungi, were recorded. Anamorphic fungi dominated both environments, contributing more than 70% of indoor and approximately 65% of outdoor fungal populations. The rainy and winter seasons showed relatively higher fungal abundance compared to summer. Dominant fungi included Cladosporium oxysporum, Cladosporium cladosporioides, Alternaria alternata, Aspergillus niger, Fusarium oxysporum, and Candida albicans. The detection of allergenic and clinically important fungi, including Candida, Aspergillus, and dermatophytes, highlights potential health risks in healthcare environments. While ANOVA revealed no significant seasonal differences, but confirmed year-round persistence. This study provides essential baseline data for aeromycological assessment in primary healthcare facilities and emphasizes the need for routine air quality monitoring and effective environmental management strategies.