AN In-vitro ANTI-INFLAMMATORY ACTIVITY OF HYDROALCOHOLIC EXTRACTS AND ESSENTIAL OIL OF SYZYGIUM AROMATICUM

Main Article Content

K. KASYAP
SINCHU YESUDANAM
M. L. LAL PRASANTH
C. R. SHIBU PRASANTH

Abstract

Objective: The need of the current study is to explore on the in-vitro anti-inflammatory activity that is the cell membrane stabilizing effect of hydroalcoholic extracts and essential oil by the crude drug Syzygium aromaticum.

Materials and Methods: In this investigation blood sample was withdrawn from healthy human subjects for the preparation of erythrocyte suspension using certain buffers. Then the prepared HRBC suspension was disclosed to essential oil of certain concentration and different graded concentrations of hydroalcoholic extracts from 100 to 1000 μg/mL. The various cytoprotective assays implemented in this research includes HRBC membrane stabilization test, heat induced and hypotonicity induced hemolysis. The standard drug used in this work is Diclofenac Sodium. All the treatment groups were carried out in a triplicate manner and the result obtained is given as Mean ± standard deviation statistically.

Results: It was observed that the in-vitro anti-inflammatory activity of EEZA showed significant result and was effective. The aqueous extracts of the crude drug also showed little anti- inflammatory property. The essential oil also was potent enough to stabilize cell membrane in unheated and isotonic induced hemolytic study at 200 µg/ml.

Conclusion: Thus the present study results explains that EEZA is effective out of all the extracts and essential oil was potent. Thereby this plant can be used in the treatment of various inflammatory ailments.

Keywords:
Syzygium aromaticum, in vitro, Diclofenac sodium, anti-inflammatory activity, EEZA

Article Details

How to Cite
KASYAP, K., YESUDANAM, S., PRASANTH, M. L. L., & PRASANTH, C. R. S. (2021). AN In-vitro ANTI-INFLAMMATORY ACTIVITY OF HYDROALCOHOLIC EXTRACTS AND ESSENTIAL OIL OF SYZYGIUM AROMATICUM. Journal of International Research in Medical and Pharmaceutical Sciences, 16(3), 33-39. Retrieved from https://www.ikprress.org/index.php/JIRMEPS/article/view/6821
Section
Original Research Article

References

Seril DN, Liao J, Yang GY, Yang CS. Oxidative stress and ulcerative colitis-associated carcinogenesis: Studies in humans and animal models. Carcinogenesis. 2003; 24(3):353-62.

Osadebe PO, Okoye FBC. Anti-inflammatory effects of crude methanolic extract and fractions of Alchornea cordifolia leaves. Journal of Ethnopharmacology. 2003;89(1):19-24.

Corley DA, Kerlikowske K, Verma R, Buffler P. Protective association of aspirin/ NSAIDs and esophageal cancer: A systematic review and meta-analysis. Gastroenterology. 2003; 124(1):47-56.

Abushouk AI, Negida A, Ahmed H, Abdel-Daim MM. Neuroprotective mechanisms of plant extracts against MPTP induced neurotoxicity: Future applications in Parkinson’s disease. Biomed. Pharmacother. 2017;85:635–645.

Abushouk AI, Ismail A, Salem AMA, Afifi AM, Abdel-Daim MM. Cardioprotective mechanisms of phytochemicals against doxorubicin-induced cardiotoxicity. Biomed. Pharmacother. 2017;90:935–946.

Ekor, M. The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Front. Pharmacol. 2014;4:177.

Cortés-Rojas DF, de Souza CR, Oliveira WP. Clove (Syzygium aromaticum): A precious spice. Asian Pac. J. Trop. Med. 2014; 4:90–96.

Batiha GES, Beshbishy AA, Tayebwa DS, Shaheen MH, Yokoyama N, Igarashi I. Inhibitory effects of Syzygium aromaticum and Camellia sinensis methanolic extracts on the growth of Babesia and Theileria parasites. Ticks Tick. Borne Dis. 2019;10:949–958.

Chomchalow N. Spice production in Asia—An overview. In Proceedings of the Conference IBC’s Asia Spice Markets 96 Conference, Singapore; 1996.

Shan B, Cai YZ, Sun M, Corke H. Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. J. Agric. Food Chem. 2005;53:7749–7759.

Martínez-Herrera A, Pozos-Guillén A, Ruiz-Rodríguez S, Garrocho-Rangel A, Vértiz-Hernández A, Escobar-García DM. Effect of 4-Allyl-1-hydroxy-2-methoxybenzene (eugenol) on inflammatory and apoptosis processes in dental pulp fibroblasts. Mediators Inflamm. 2016;2016:9371403.

Miyazawa M, Hisama M. Suppression of chemical mutagen-induced SOS response by alkylphenols from clove (Syzygium aromaticum) in the Salmonella typhimurium TA1535/pSK1002 umu test. J. Agric. Food Chem. 2001;49:4019–4025.

Jirovetz L, Buchbauer G, Stoilova I, Stoyanova A, Krastanov A, Schmidt E. Chemical Composition and Antioxidant properties of clove leaf essential oil. J. Agric. Food Chem. 2006;54:6303–6307.

Tanko Y, Mohammed A, Okasha MA, Umar AH, Magaji RA. Anti-Nociceptive and Anti-Inflammatory Activities of Ethanol Extract of Syzygium Aromaticum Flower Bud in Wistar Rats and Mice. 2008;5(2):209–212.

Pharmacopée française, Maisonneure SA. éditeur, 10th edition; 1985.

Chioma AA, Onyechi O, Lawrence USE. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU. 2012;20(1):76.

Seema CC, Sharan SV, Srinivasa RB, Meena V. In vitro antiinflammatory activity of methanolic extract of Centella asiatica by HRBC membrane stabilisation. Rasayan J Chem. 2011;4(2):457-460.

Sirisha JV, Sailakshmi K, Vijay K. Human red blood cell (HRBC) membrane stabilizing activity of leaves of petether extract of Vanda tessellata Rox. International Ayurvedic Medical Journal. 2013;1(6):5-8.