INVESTIGATING THE SYNERGISTIC EFFECTS OF HABITUAL CHEMICALS ON Lumbricus terrestris ACTION POTENTIALS

Main Article Content

JOSHUA J. YOON
JONGBIN LEE

Abstract

The chemicals that an individual regularly craves are described as habitual. Alcohol, caffeine, and nicotine are some of the most popular habitual substances globally, and these substances are being consumed at an increasing rate in younger ages. In Europe and North America, up to 90% of the adult population drinks coffee daily, and, although less prevalent, other drugs are also used simultaneously in combination. Moreover, 37.3 percent of high school seniors reported that they were smoking. Many documented health issues for each of these chemicals have been identified regarding decreased brain function and even permanent brain damage. Moreover, these chemicals are often utilized concurrently, which may lead to different responses by the brain. The study aimed to examine the synergistic effects on action potential parameters acquired from the nerve cord of Lumbricus terrestris by injecting mixed solutions of alcohol, caffeine, and nicotine.

The controlled amounts of alcohol, caffeine, and nicotine solutions were administered into the ventral nerve cord of L. terrestris under anesthesia, and the action potentials were induced with electric stimulation and their derived parameters of action potential such as latent period, wave width, peak point, and trough point were evaluated.   The data demonstrated the reliability of the model's parameters by consistent change of regression slopes from action potentials.   The observable trends in the mean latent period, peak point, trough point, and action potential length for greater effects were proven to be synergistic when the combination of the three drugs was administered.

Keywords:
Action potential, habitual chemicals, psychoactive drugs, Lumbricus terrestris, nervous system

Article Details

How to Cite
YOON, J. J., & LEE, J. (2021). INVESTIGATING THE SYNERGISTIC EFFECTS OF HABITUAL CHEMICALS ON Lumbricus terrestris ACTION POTENTIALS. Journal of International Research in Medical and Pharmaceutical Sciences, 16(1), 34-46. Retrieved from https://www.ikprress.org/index.php/JIRMEPS/article/view/6393
Section
Original Research Article

References

American Addiction Centers, Habitual drug use, AlcoholRehab.com; 2020.
Available:https://alcoholrehab.com/drug-addiction/effects/habitual-drug-use/.

Basu Sutapa, Deeptanshu Basu. The relationship between psychoactive drugs, the Brain and Psychosis, International Archives of Addiction Research and Medicine. 20151;1.
ISSN: 2474-3631.
Available:clinmedjournals.org/articles/iaarm/iaarm-1-003.pdf

Hohmann Nicolas, et al. Effects and risks associated with novel psychoactive substances. Deutsches Arzteblatt International. 2014;111(9):139-147.
Available:www.ncbi.nlm.nih.gov/pmc/articles/PMC3965957/

Ron Weathermon, David W Crabb. Alcohol and medication interaction, Alcohol Research & Health. 1999;23(1):40-55.

Abernathy Kenneth, et al. Alcohol and the prefrontal cortex. International Reviews on Neurobiology. 2013;90:289-320.
www.ncbi.nlm.nih.gov/pmc/articles/PMC3593065/

Richard J Bonnie, Kathleen Stratton, Leslie Y Kwan. Public health implications of raising the minimum age of legal access to tobacco products.” Book Chapter in Tobacco Products, edited by Richard J Bonnie et al.; 2015.
Available:www.ncbi.nlm.nih.gov/books/NBK310412/

Jiloha RC. Biological basis of tobacco addiction: implications for smoking-cessation treatment. Indian Journal of Psychiatry.2010;52(4):301-307.
www.ncbi.nlm.nih.gov/pmc/articles/PMC3025154/

Temple Jennifer L, et al. The Safety of ingested caffeine: A comprehensive review. Front Psychiatry. 2017;8(80).
Available:www.ncbi.nlm.nih.gov/pmc/articles/PMC5445139/

Shen Hai-Ying, Jiang-Fan Chen. Adenosine A2A receptors in psychopharmacology: Modulators of behavior, mood, and cognition, Current Neuropharmacology. 2009;7(3):195-206.
Available:www.ncbi.nlm.nih.gov/pmc/articles/PMC2769003/

Tallarida Ronald J. Quantitative Methods for Assessing Drug Synergism, Genes Cancer. 2011;2(11):1003-1008.
Available:www.ncbi.nlm.nih.gov/pmc/articles/PMC3379564/

Jeannine T Abiva, Erika T Camacho, Edna S Joseph, Arpy K Mkaelian, Charles R Rogers, John Shelton, Stephen A Wirkus. The effect of alcohol on neuron firing, Math. Scientist. 2007;32:32-40.

Matthew Brothers R, Kevin M Christmas, Jordan C Patik, Paul S Bhella. Heart rate, blood pressure and repolarization effects of an energy drink as compared to coffee, Clinical Physiology Functional Imaging. 2017;37(6):675-681.

Dominik H Pesta, Siddhartha S Angadi, Martin Burtscher, Christian K Roberts. The effects of caffeine, nicotine, ethanol and tetrahydrocannabinol on exercise performance, Nutrition and Metabolism. 2013;10:71:1- 15.

Quiroga Sigmer, et al. Evolution of flatworm central nervous systems: Insights from polyclads, Molecular Biology. 2015;38(3):233-248.

Holland Linda Z. The origin and evolution of chordate nervous systems, Philos Trans R. Soc Lond B. Biolo Sci. 2015;370(1684).
Available:www.ncbi.nlm.nih.gov/pmc/articles/PMC4650125/

Aske Krogsgaard. Physics of nerves: Action potentials in Lumbricus terrestris axons in the presence of anesthetics and the collision of pulses, Department of Physics and Astronomy, University of Aarhus; 2016.
Available:https://www.nbi.ku.dk/membranes/thesis-pdf/2016_Master_AskeKrogsgaard_small.pdf

Amir H Rezvani, Hannah G Sexton, Joshua Johnson, Cori Wells, Karen Gordon, Edward D. Levin, Effects of caffeine on alcohol consumption and nicotine self-administration in rats, Alcohol Clin Exp Res. 2013;37(9):1609-1617.

Hyunseo Ariel Kim, Sungwon Cho, Yu Jin Cho, Juhun Han, Andrew Hwang, Anthony W. Bae, Gi Won Sung, Taehyuk Kim and Jongbin Lee, Investigating pharmacological effects on action potentials form Lumbricus terrestris nerve cords, Journal of International Research in Medicine and Pharmaceutical Sciences. 2018;13(3):119-130.

Gamry Instrument. Technical Note: The Faraday cage: What is it? How does it work?, Gamry Instrument, Inc.
Available:https://www.gamry.com/assets/Application-Notes/Faraday-Cage.pdf

Imad Kobeissl. Noise reduction techniques for microcontroller-based systems, Freescale Semiconductor, Inc; 2004.
Available:https://www.nxp.com/docs/en/application-note/AN1705.pdf

Kyle M. Shannon, Gregory J Gage, Aleksandra Jankovic, Jefferey Wilson W, Timothy C Marzullo. Portable conduction velocity experiments using earthworms for the college and high school neuroscience teaching laboratory, Adv. Physiol. Educ. 2014;38(1):62-70.

Nikolay Kladt, Ulrike Hanslik, Hans-Greorg Heinzel. Teaching basic neurophysiology using intact earthworms, Journal of Undergrad Neurosci Edu. 2010;9(1):A20-A35.

Charles Heller, Kevin Crisp. A Hodgkin-Huxley model for conduction velocity in the medial giant fiber of the earthworm, Lumbricus terrestris, St. Olaf College, Northfield, Minnesota. 2016;1-9.

Swaroop Chand Bhansali, Ragam Ravi Sunder, Perugu Balananda, Venkateswarlu K, Parvathi R. Nerve conduction studies in asymptomatic alcoholics, Journal of Medical Science and Clinical Research. 2015;3(6):6347-6352.

Roberta Donato, Marco Canepari, Remigijus Lape, Andrea Nistri. Effects of caffeine on the excitability and intracellular Ca(2+) transients of neonatal rat hypoglossal motoneurons in vitro, Neuroscience Letters. 2003;346(3):177-181.

Timothy Lund, Caitlin Monroe, Savannah Seaver. The effects of nicotine dosage on action potential conduction velocity of the medial giant neuron in the Lumbricus terrestris, Research Gate. DOI: 10.13140/RG.2.2.22732.46721, 2018

Marc-Antoine Crocq. Alcohol, nicotine, caffeine, and mental disorders, Dialogues Clinical Neuroscience. 2003;5(2):175-185.

Concepcion Vinader-Caerols, Santiago Monleon, Carmen Carrasco, Andres Parra. Effects of alcohol, coffee, and tobacco, alone or in combination, on physiological parameters and anxiety in a young population, Journal of Caffeine Research. 2012;2(2):70-76.