THE USE OF PHOSPHOMOLIBDATE METHOD FOR DETERMINATION OF ANTIOXIDANT ACTIVITY IN MILK EXTRACT

Main Article Content

ILMIJE VLLASAKU
JULIJANA TOMOVSKA

Abstract

Antioxidants are mainly non-nutrient compounds in both human and animal feed, but have the antioxidant capacity in vitro to provide an artificial power index in preventing the destruction of cells and tissue potential by inhibiting nutrient oxidation. Milk contains lipophilic and hydrophilic antioxidants, which play a key role in maintaining. The efficiency of extraction for determining the antioxidant activity of milk corresponds to the method used for plant extraction and it is in a strong linear positive correlation. For this purpose, the phosphor molybdenum method based on the reduction of Mo (VI) in Mo (B) in samples of milk. Extracts obtained with 6% trichloroacetic acid (TCA). Green complexes formed at acidic pH value and measurement spectrophotometric in the UV range at wavelength λ = 695 nm. The values ​​of the milk samples applied to the gallic acid calibration curve. For the antioxidant activity, four types of milk tested with 10 extracts for each sample and statistics made in Excel. The results were lower in line with standard commercial milk. The conclusion is that the highest value of antioxidant activity is in packaged milk with 3.2% fat, which is due to the effects of fats, the presence of vitamin E and their synergistic effect with vitamin C. On the other hand, we conclude that the highest value of the total antioxidant activity in milk, the first sample is due to the use of several types of animal feed such as alfalfa, two concentrates and straw, which proves the dependence of antioxidant activity on the impact of nutrients, ie their type and quantity.

Keywords:
Antioxidants, antioxidant activity, phosphomolibdate method, cow milk

Article Details

How to Cite
VLLASAKU, I., & TOMOVSKA, J. (2021). THE USE OF PHOSPHOMOLIBDATE METHOD FOR DETERMINATION OF ANTIOXIDANT ACTIVITY IN MILK EXTRACT. Journal of Applied Chemical Science International, 12(2), 10-17. Retrieved from https://www.ikprress.org/index.php/JACSI/article/view/7113
Section
Original Research Article

References

Irshad M, Chaudhuri PS. Oxidant-antioxidant system: role and significance in the human body. Department of Laboratory Medicine, New Delhi, India. Indian Journal of Experimental Biology. 2002;40(11):1233-9.

Pokorny J, Korczak J. Preparation of natural antioxidant. In: Pokorny J, Yanishlieva N, Gordon M, editors, Antioxidants in Food: Practical Applications. Woodhead Publishing Limited, Abington, Cambridge, England. 2001;311-330.

Carlsen HМ, Bente L, Halvorsen KH, Bohn SK, Dragland S, Sampson L, Willey C, Senoo H, Umezono Y, Sanada C, Barikmo I, Berhe N, Walter M.C, Phillips W, Jacobs K.Jr. D.R. and Rune B. The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used world wide. Nutr J, Published: 22 January 2010.

Badarinath AV, RAo KM, Chetty CMS, Ramkanth V, Rajan TVS, Gnanaprakash K. A review on in-vitro antioxidant methods: comparisons, correlations and considerations. Int. J. Pharm Tech Res. 2010;2(2): 1276–1285.

Mavromichalis I. Evaluating the importance of antioxidants in feed. Animal nutrition, Feed ingridients, WATT AgNet com, Famsun; Phileo Lesafre Animal Care; 2012.

Theodoridou K, Koidis T. New approaches to feeding dairy cows: The use of bioactive compounds from plant and food resources, FindAPhD Project. (Queen’s University Belfasт, European/UK); 2015-2017.

Grażyna C, Czeczot H, Ambroziak A, Bielecka MM. Natural antioxidants in milk and dairy products. International Journal of Dairy Technology. May 2017;70(2):165-178.

Kamal-Eldin A, Appelquist LA. The chemistry and antioxidant properties of tocopherols and tocotrienols [1996] Lipids, ISSN: 0024-4201, In AGRIS since: 2013;31:731:671-701.

Palozza P, Calviello G, Maggiano N, Lanza P, Ranelletti F.O, Bartoli G. M. Beta-carotene antagonizes the effects of eicosapentaenoic acid on cell growth and lipid peroxidation in WiDr adenocarcinoma cells. Free Radic. Biol. Med. 2000;28:228-234.

Gjorgievski N, Tomovska J, Dimitrovska D, Makrijoski B, Shariati MA. Determination of the antioxidant activity in yogurt; Journal of Hygienic Engineering and Design JHED. 2014;8:88-92. UDC 637.146.3:615.272.

Tomovska J, Gjorgievski N, Makarijoski B. Examination of pH, Titratable Acidity and Antioxidant Activity in Fermented Milk, Journal of Materials Science and Engineering A. 2017;6(11-12):326-333.

Tomovska J, Menkovska M, Ahmad MA. Determination of vitamin C in different types of milk, The International Journal of Engineering and Science (IJES). 2018;7(5):ver. 2:77-82.

Tomovska J, Vllasaku I. Phosphomolibdate test method for antioxidant activity in extracts of animal feed. Proceeding book of abstracts, III Balkan Agriculture Congress, Edirne, Turkey, 29 August - 1 September, 2021;650-659. ISBN: 978-975-374-299-3 Trakya University Publisher: 254.

Alam N. Md, Nusrat JB, Rafiquzzaman Md. Review on in vivo and in vitro methods. Saudi Pharm. Journal. April 2013;21(2):143–152 . DOI: 10.1016/j.jsps.2012.05.002

Preto P, Pineda M, Aguilar. M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry. June 1999;269(2):337-41. DOI: 10.1006/abio.1999.4019

Hayajneh F, Firas M. Antioxidants in Dairy Cattle Health and Disease. Bulletin UASVM Veterinary Medicine, 2014;71 (1):104-109 Print ISSN 1843-5270; Electronic ISSN 1843-5378.

Cornell C, Cornell Universitety. College of Agriculture and Life Sciences, Dairy Extension, Food Safety Laboratory and milk quality Improvement program, Composition of milk. 2012;1-5.

Fennema, O. Fennema's Food Chemistry. CRC Press Taylor &Francis. 2008;454-455.

Hurley W.L. Milk Composition and Characteristics, Milk composition & Synthesis, University Illinois, Urbana,Champaign; 2009.

Milosavljevic ZM, Pauca M. Livestock, Agriculture review. Beograd; 1978.