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This research investigated the effect of pesticides application on the growth of two soil nitrifying bacteria species, Nitrosomonas and Nitrobacter respectively. The bacteria were isolated from soil samples obtained from Bingham University, Karu local government area of Nassarawa State, Nigeria. The soil bacteria were cultured using the Manniol Yeast Extract Agar (MYEA). The bacteria isolates where identified and characterized based on their morphology and chemical characteristics. The growth count of the bacteria after four (4) days isolation showed increase in the bacteria growth. Nitrosomonas obtained from MYEA ranged from 23 to 102 (x105) cfu/g, and the growth of Nitrobacter count obtained from MYEA ranged from 24 to 97 (x105) cfu/g. there was an increase in bacteria growth at both control level and decrease in bacteria growth at concentration level of 20% to 100% within 120 hours of observation for Nitrosomonas species. The result shows that F_ cal 0.830 was greater than F-tab 0.05, and Nitrobacter species. F_ cal 1.360 was greater than f-tab 0.05. Since the F- cal value is greater than F- tab value for both the Nitrosomonas and Nitrobacter species. The Null hypothesis is rejected and alternate is accepted. This shows that the nitrifying bacteria could not survive or grow under high pesticides concentration. It is therefore very important to examine pesticides in order to determine it toxicity to soil bacteria before application.

Soil, bacteria, pesticides, growth and isolates

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How to Cite
IHUMA, J. O., PHILIP, A., & KURAH, B. E. (2021). EFFECT OF PESTICIDE (CHLORYPYRIPHOS) ON THE GROWTH OF NITRIFYING BACTERIA (Nitrosomonas AND Nitrobacter species) ISOLATED FROM THE SOIL. Asian Journal of Plant and Soil Sciences, 6(1), 233-238. Retrieved from https://www.ikprress.org/index.php/AJOPSS/article/view/7202
Original Research Article


Willey JM, Sherwood LM, Woolverton C. Prescott’s microbiology. 8th Ed. McGraw-Hill Companies. New York; 2011.

Scragg A. Environmental biotechnology. 2nd, Ed. Oxford University Press. New York; 2005.

Bona C, Mendonca de Rezende I, Gedir de Oliveira S, Antonio de Souza L. Effect of soil contaminated by diesel oil on the germination of seeds and the growth of (Anacardiaceae) Sellings. Brazilian Archives of Biology and Technology. 2011;54 (6):77 – 82.

DeLorenzo ME, Scott GI, Ross PE. Toxicity of pesticides to aquatic microorganisms: a review. Environmental. Toxicology. Chem. 2001; 20(1): 84-98.

Lopez L, Pozo C, Rodelaz B, Calvo C, Juarez B, Martinez-Toledo MV, Gonzalez-Lopez J. Identification of bacteria isolated from an oligotrophic lake with pesticide removal capacities. Ecotoxicology. 2005;14:299-312.

NPC. National Population Commission. Federal Republic of Nigeria Official gazette, Lagos, Nigeria. Federal Government Printer; 2006.

Ferrari BC, Binnerup SJ, Gillings M. Microcolony cultivation on a soil substrate membrane system selects for previously uncultured soil Bacteria. Journal of Applied Environmental Microbiology. 2005;71:8714-870.

Denning EH, Day TA, Oquist G. Effects of soil temperature. J Experiment Bot. 2005; 42:611-617.

Udochukwu U, Dave-Omeregie AO, Akaluzia. Effect of pesticide on bacteria growth. Journal of Application. Science Environmental Management. 2018;22 (3):304-307.

Cheesbrough Monica. District Laboratory practice in tropical countries. 2005;8(4):90 – 100.

Atuanya EI, Dave-Omoregie AO, Udochukwu U, Inetianbor J. Toxicological effects of plastic composted soil on nitrifying bacteria. British Biotechnology Journal. 2016;13 (4): 1-7.

Gupta G, Kelly P. Poultry litter toxicity comparison from various bioassays. Journal of Environmental Science and Health. 2009;22(4): 1083-1093.

Haunstra L, Dollman P, Voshaar JHO. The use of sig modial dose response curves in the soil ecotoxicological research. Plant Science. 2007;84:293-297.

Okpokwasili GC, Odokuma LO. Tolerance of nitrobacter to toxicity pf some Nigerian crude oils. Bullentin of Environental Contamination and Toxicity. 1994;52:388-395.