EFFECT OF PRE AND POST CULTURE TEMPERATURE TREATMENTS ON DIRECT MICROSPORE EMBRYOGENESIS IN INDIAN CHILLI PEPPER (Capsicum annuum L.)

Main Article Content

H. M. ARJUN
BANDARI RAJESH
SRIPATHI VIJAY GOUD
S. LOKESHA
P. SATEESH KUMAR

Abstract

The present study was conducted to determine the effect of the pre and post temperature treatment on embryo induction and green plant regeneration from chilli anther culture and how it is influenced by low temperature treatments. Microspore embryogenesis is an efficient method to produce double haploids in Indian Chilli pepper. The effect of androgenesis for 10 F1 genotypes was evaluated in two different temperature treatments. All the genotypes showed differential expression for two pre cold treatments (fresh, 1 Day cold treatment and 2 Days cold treatment) and post culture cold treatment at 9°C for 8 days. Highest frequency of direct embryogenesis for different genotypes varied between 37.5% to 4.13% in PH53 and PH17 respectively, at 2 days pre cold treatment and 9°C post culture cold treatment. Both pre and post temperature treatment played an important role in direct embryogenesis. Highest rate of embryo induction rate was observed in 2 days cold treatment and 9°C post culture treatment across the genotypes. This clearly indicates pre and post cold temperature treatments has direct influence on frequency of androgenesis.

Keywords:
Embryo, anther culture, haploid, induction, direct microspore embryogenesis.

Article Details

How to Cite
ARJUN, H. M., RAJESH, B., VIJAY GOUD, S., LOKESHA, S., & SATEESH KUMAR, P. (2020). EFFECT OF PRE AND POST CULTURE TEMPERATURE TREATMENTS ON DIRECT MICROSPORE EMBRYOGENESIS IN INDIAN CHILLI PEPPER (Capsicum annuum L.). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(1-2), 49–58. Retrieved from http://www.ikprress.org/index.php/PCBMB/article/view/4923
Section
Original Research Article

References

Morrison RA, Koning ER, Evans DA. Pepper. In: Handbook of plant cell culture. Macmillan, New York. 1986a;4:552–573.

Supena ED, Suharsono S, Jacobsen E, Custer JB. Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.). Plant Cell Rep. 2006;25:1–10.

Hand ML, Koltunow AM. The genetic control of apomixis: Asexual seed formation. Genetics. 2014;97:441–450.

Grusz AL. A current perspective on apomixis in ferns. J. Syst. Evol. 2016; 54:656–665.

Dumas de Vaulx R, Pochard E. Parthenogenese et androgenese chezlepiment. Role actuel dans les programs de selection. Le Selectionneur Francais. 1986;36:1-12.

Murashige T, Skoog FA. Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473–497.

Arjunappa HM, Sateesh Kumar P, Premalatha D. Studies on ploidy analysis and chromosome doubling in androgenic plants of chilli pepper (Capsicum annuum L.). International Journal of Agriculture Innovations and Research. 2016;4:627-674.

Touraev A, Heberle-Bors E. Initiation of embryogenesis by stress. Trends Plant Science. 1997;2(8):297-302.

Shariatepanahi ME, Vyur Bal, Heberle-Bors E, Touraev A. Stress applid for the reprogramming of plant microspores towards in vitro embryogenesis. Physiologia Plantarum. 2006;127:519-534.

Nitsch C, Norreel B. Effete d’un choc thermique sur le pouvoirembryogene du pollen de Datura innoxiacultive dans 1’anthere ou isle de 1’anthere. Comptes Rendus de 1’ Academie des Sciences Paris. 1973;276:303-306.

Sunderland N, Evans LJ. Multicellular pollen formation in cultured barley anthers. Journal of Experimental Botany. 1980; 31(2):501-514.

Hu TC, Kasha KJ. A cytological study of pretreatment used to improve isolated microspore cultures of wheat (Triticum aestivum L. cv Chris). Genome. 1999; 42(3):432-441.

Sunderland N, Roberts M, Evans LJ, Wildon DC. Multicellular pollen formation in cultured barley anthers. Journal of Experimental Botany. 1979;30(6):1133-1144.

Xie JH, Gao MW, Liang ZQ, Shu QY, Cheng XY, Zue QZ. The effect of cold-pretreatment on the isolated microspore culture and the free amino acid change of anthers in japonica rice (Oryza sativa L.). Journal of Plant Physiology. 1997;151(1): 79-82.

Sibi M, Dumas de Vaulx R, Chambonnet D. Obtaining haploid plants by in vitro ndrogenesis in red pepper (Capsicum annuum L.). Annales de l’Amelioration des Plantes. 1979;29:583–606.

Morrison RA, Koning ER, Evans DA. Anther culture of an inter specific hybrid of Capsicum. J Plant Physiol. 1986b;126:1–9.

Ozkum D, Tipirdamaz R, Ellialtioglu S. The relationship between the endogenous abscisic acid content of anthers and in vitro androgenesis in pepper (Capsicum annuum L.). Acta Hortic. 2001;560:327-329.

Touroev A, Pfosser M, Vicente O, Heberle-Bors E. Stress as the major signal controlling the developmental of tobacco microspores: Towards a unified model of induction of microspore/pollen embryogenesis. Planta. 1996;200(1):144-152.

Fassauliotis G, Newlson BV. Regeneration of tetraploid Muskmelon from cotyledons and their morphological differences from two diploid Mushmelon genotypes. J Am Soc Hortic Sci. 1992;117:863- 866.

Mozafari J, Wolyn DJ, Ali-Khan ST. Chromosome doubling via tube disc culture in dihaploid potato as determined by confocal microscopy. Plant Cell Reprod. 1997;16:329-333.

Ghanavati F, Mozafari J, Masumi AA. Determination of ploidy level with counting the chloroplast number in stomatal guard cells in Medicago sp. Seed and plant Journal. 2004;20:117-127.

Dolezel J, Binarova P, Lucretti S. Analysis of nuclear DNA content in plant cells by flow cytometry. Biol Plant. 1989;31:113–120.

Lanteri S, Portis E, Bergervoet HW, Groot SPC. Molecular markers for the priming of pepper seeds (Capsicum annuum L.). J Hort Sci Biotechnol. 2000;75:607– 611.

Mityko J, Fari M. Problems and results of doubled haploid plant production in pepper (Capsicum annuum L.) via anther and microspore culture. Acta Hortic. 1997;447: 281–287.

Dumas de vaulx R, Chambonnet D, Pochard E. In vitro anther culture in red pepper (Capsicum annuum L.): Improvement of the rate of plant production in different genotypes by treatments at 35C. Agronomie. 1981;1:859-864.