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Triploids are the most important materials for the selection of citrus seedless cultivars. Moreover, flow cytometry has been used for plant DNA content estimation; likewise, it was succeeded in the use for testing ploidy levels and hereditary origin of the triploid plants. The current study’s purpose was to produce triploid hybrid from underdeveloped seeds cultivated in different media of two oranges cultivars: Pineapple and Parson Brown. For that, all abnormal seeds collected from mature fruits were sterilized and cultured on Murashige and Tucker base medium supplemented with different concentrations of composition. Then, the ploidy levels of seedlings from the rescue of immature embryos were evaluated by flow cytometry. Germination rate is the highest in M3 (MT+ 25 mg/l Adenine sulfate) with 100% and 90% for both Pineapple and Parson Brown varieties, respectively. In addition, the germination time is shorter in M1 (MT + 1 mg/l gebberellic acid) for both varieties, which takes six and seven days. The triploidy rate varied according to the seed shape. It was found in small seeds with 66%, followed by 40% in flat seeds for Parson Brown variety. While 25% were recovered from flat seeds in Pineapple cultivar. Therefore, M1 remains the best to promote high rate of germination. Flow cytometry analysis could be used for obtaining accurate and rapid results for cytological observations of seedling populations of Citrus.
Gianni Barcaccia, Diego Pessina, Silvia Nicole, Rodolfo Gentili, Graziano Rossi, Silvia Barbesti, Sergio Sgorbati. Molecular analysis and flow cytometry evaluation of nuclear DNA in Citrus limonimedica Lush, to assess genetic variability and to trace phylogenetic relationship among lemon and cedar. Cytometry Part A. 2008;73:67.
Talent N, Dickinson TA. Polyploidy in Crataegus and Mespilus (Rosaceae, Maloideae): Evolutionary inferences from flow cytometry of nuclear DNA amounts. Can. J. Bot. 2005;83:1268-1304.
Bannet MD, Leitch IJ. Nuclear DNA amounts in angisperms. Ann Bot. 1995;76:113-176.
Brown SC, Bergounioux C. Plant flow cytometry. Advanced Research and Clinical Applications. Yen A, Ed. Boca Raton, Florida: CRC.Press. 1989;2.
Nosrati H, Price AH, Gerstberger P, Wilcock CC. Identification of a natural allopentaploid hybrid Fragaria (Rosaceae), new to Europe. New J. Bot. 2011;1:88-92.
Seker M, Tuzcu O, Ollitrault P. Comparison of nuclear DNA content of citrus rootstock populations by flow cytometry analysis. Plant Breeding. 2003;122:169–172.
Vrána J, Cápal P, Bednářová M, Doležel J. Flow cytometry in plant research: A success story. In: Applied Plant Cell Biology. Plant Cell Monographs. Nick P, Opatrny Z (Eds), Springer, Berlin, Heidelberg, Germany. 2014;22:395-430.
Sliwinska E, Zielińska E, Jędrzejczyk I. Are seeds suitable for flow cytometric estimation of plantgenome? Cytometry A 64A. 2005;72-79.
Suda J, Trávnícek P. Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry – new prospects for plant research. Cytometry A. 2006;69A:273-280.
Razafinarivo NJ, Rakotomalala JJ, Brown SC, Bourge M, Hamon S, de Kochko A. Geographical gradients in the genome size variation of wild coffee trees (Coffea) native to Africa and Indian Ocean islands. Tree Genet. Genomes. 2012;8:1345-1358.
Ollitrault P, Michaux- Ferriere N. Application of ﬂow cytometry for Citrus genetics and breeding. Proc. Int. Soc. Citr. 1992;1:193-198.
Jia-Long Yao, Daniel Cohen. Production of triploid Zantedeschia hybrids using embryo rescue. New Zealand Journal of Crop and Horticultural Science. 1996;24(4):297-301.
Mahmoudi K, Handaji N, Arsalane N, Ibriz M, Aderdour T, Label K, Ait El Aouad B, Benyahya H. Preliminary selection of the orange cultivars using as female parents in diploids crosses and in triploidy program. International Journal of Botany Studies. 2017;2(6):232-240.
Handaji Najat, Benyahia Hamid, Arsalane Najat, Benaouda Hassan. AYA and HANA: New seedless mandarin’s triploid hybrids selected in Morocco. Biotechnology Journal International. 2018;21(1):1-9.
Mohsen Moussavi Nik, Mahdi Babaeian, Abolfazl Tavassoli. Effect of seed size and genotype on germination characteristic and seed nutrient content of wheat scientific research and essays. Academic Journals. 2011;6(9):2019-2025.
DOI: 10.5897/SRE11.621 ISSN 1992-2248
Willenborg CJ, Wildeman JC, Miller AK, Rossnaged BG, Shirtliffe SJ. Oat germination characteristics differ among genotypes, seed sizes, and osmotic potentials. Crop Sci. 2005;45:2023-2029.
Baker HG. Seed weight in relation to environmental conditions in California. Ecology. 1972;53:997–1010.
Silvertown JW. Seed size, life span, and germination date as coadapted features of plant life history. Am. Nat. 1981;118:860–864.
Stanton ML. Seed variation in wild radish: Effect of seed size on components of seedling and adult fitness. Ecology. 1984;65:1105–1112.
Gross KL. Effects of seed size and growth form on seedling establishment of six monocarpic perennial plants. J. Ecol. 1984;72:369–387.
Hendrix SD. Variation in seed weight and its effect on germination in Pastinaca sativa L. (Umbelliferae). Am. J. Bot. 1984;71:795–802.
Singh ND. Seed size and adventitious (nodal) roots as factors influencing the tolerance of wheat to waterlogging. Australian J. Agric. Res. 2003;54:969-977.
Arunachalam A, Khan ML, Singh ND. Germination, growth and biomass accumulation as influenced by seed size in Mesua ferrea L. Turkish J. Bot. 2003;27:343-8.
Kaydan D, Yağmur M. Germination, seedling growth and relative water content of shoot in different seed sizes of triticale under osmotic stress of water and NaCl. Afr. J. Biot. 2008;7:2862-2868.
Larsen SU, Andreasen C. Light and heavy seeds differ in germination percentage and mean germination thermal time. Crop Sci. 2004;44:1710-1720.
Gray D, Steckel JRA. The effects of ripening and drying of carrot seed (Daucus carrota) crops on germination. Ann Appl. Biol.1982;101:346-347.
Ninh TP, Hiroshi N, Toru T. Effect of umbel order and umbellet position on the production and characteristics of seeds and on the development and growth of seedlings in Angelica acutiloba Kitagawa. Jpn. J. Trop. Agr. 2007;51:46-53.
Harper JL. Population biology of plants. Academic, London; 1977.
Jaskani MJ, Abbas H, Khan MM, Shahzad U, Hussain Z. Morphological description of three potential citrus rootstocks. Pak. J. Bot. 2006;38:311-318.
Guo F, Zhang JR, Chen SC. Production of triploid plantlets by In vitro culture of citrus embryos. J. Hered. 1988;10:9-11.
Phanna Phat, Sameena Sheikh, Jeong Hyeon Lim, Tae Bok Kim, Mun Ho Seong, Hyong Gwon Chon, Yong Kyu Shin, Young Ju Song, Jaejong Noh. Enhancement of seed germination and uniformity in triploid watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai). Korean J. Hortic. Sci. Technol. 2015 ;33(6):932-940. ISSN: 1226-8763.
Geraci G, Esen A, Soost R. Triploid progenies 2x X 2x crosses of Citrus cultivars. J. Hered. 1975;66:177-178.
Aleza P, Juárez J, Ollitrault P, Navarro L, Bot A. Polyembryony in Non-apomictic Citrus genotypes. Annals of Botany. 2010;106(4):533-45.
Viloria Z, Grosser JW, Bracho B. Immature embryo rescue, culture and seedling development of acid citrus fruit derived from interploid hybridization. Plant Cell, Tissue and Organ Culture. Springer. 2005;82:159-167.
Cushman KE, Horgan TE, Snyder RG, Hudson PM, Coker CH, Ely M. Evaluation of elongated and oval triploid (seedless) watermelon genotypes. Annual Report 2002 of the North Mississippi Research & Extension Center. Mississippi Agriculture & Forestry Experiment Station Information Bulletin. 2003;398:339-345.
Aleza P, Juárez J, Cuenca J, Ollitrault P, Navarro L. Recovery of citrus triploid hybrids by embryo rescue and flow cytometry from 2x × 2x sexual hybridisation and its application to extensive breeding programs. Plant Cell Rep. 2010;29:1023-1034.
Esen A, Soost RK. Seed development in Citrus with special reference to 2× × 4× crosses. American Journal of Botany. 1973;60:448-452.