Main Article Content
Trichoderma is used as a bio-agent and have been reported to be quite effective, inexpensive and eco- friendly. It can be used as seed treatment, applied direct to the soil before planting and added to organic fertilizers. Trichoderma controls the pathogenic organism by competition, mycoparasitism and antagonism. It excretes enzymes like viridin and gleotoxin thereby enhancing the root growth. Hence, it has got significant importance in ecofriendly disease management programs. The first step while studying the bio-control, is the identification of a promising bio- control agent. Once the bio-control agent is identified and is proven effective against plant pathogens over several reproducible results, the method of mass production, formulation and application should be taken into consideration to stabilize the product during storage and to facilitate its delivery to the plant. The major hurdle in the use of bio-pesticides is their shelf life and inconsistent performance. Therefore, the present study “Shelf life studies of different formulations of Trichoderma harzianum” was undertaken at biological control lab, NIPHM Hyderabad with the objectives: Evaluation of solid and liquid substrates for mass production of T. harzianum and Shelf life studies of different formulations of T. harzianum. From the study it was found that all the prepared formulations retained optimum viability. Capsule and sachet based formulations gave higher shelf life of Trichoderma as compared to wettable formulations. Application of Capsule and sachet based formulations would be more convenient for application, storage and handling to control diseases in field and orchards which in turn would help the farmers to achieve a better crop yield.
Harman GE. Seed treatments for biological control of plant disease. Crop Prot. 1991; 10:166–171.
Howell CR. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Disease. 2003;87:4–10.
Jin X, Harman GE, Taylor AG. Conidial biomass and desiccation tolerance of Trichoderma harzianum produced at different medium water potentials. Biol. Control. 1991;1:237–243.
Papavizas GC. Trichoderma and gliocladium: Biology, ecology and potential for biocontrol. Ann. Rev. Phytopathol. 1985;23:23-54.
Kolombet LV, Zhigletsova SK, Kosareva NI, Bystrova EV, Derbyshev VV, Krasnova SP, Schisler D. Development of an extended shelf-life, liquid formulation of the biofungicide Trichoderma asperellum. World Journal of Microbiology and Biotechnology. 2008;24(1):123–131.
Sriram S, Panchakshari K, Maheshwarappa R, Savitha J. Extended shelf-life of liquid fermentation derived talc formulations of Trichoderma harzianum with the addition of glycerol in the production medium. Crop Protection. 2011;30(10):1334-1339.
Khan S, Bagwan NB, Iqbal MA, Tamboli RR. Mass multiplication and shelf life of liquid fermented final product of Trichoderma viride in different formulations. Advances in Bioresearch. 2011;2(1):178–182.
Prasad RD, Rangeshwaran R. Shelf life and bioefficacy of Trichoderma harzianum formulated in various carrier materials. Plant Disease Research. 2000;15(1):38-42.
Rai D, Tewari AK. Shelf life studies of different formulations based on Trichoderma harzianum (Th14). Annals of Biological Research. 2016;7(7):1-5.
Akshata LT, Guldekar DD, Potdukhe SR, Kale SS, Kumar A. Shelf life study and antagonistic activity of Trichoderma viride in different oil formulations. Int. J. Curr. Microbiol. App. Sci. 2018;7(11):225-230.
Sriram Maya N, Guldekar DD, Potdukhe SR, Parbat JM. Assessment of shelf life of Trichoderma viride on different liquid formulations. International Journal of Current Microbiology and Applied Sciences. 2018;(Special Issue6):2575-2579.
Prasad RD, Rangeshwaran R, Anuroop CP, Phanikuma RPR. Bioefficacy and shelf life of conidial and chlamydospore formulations of Trichoderma harzianum Rifai. J. BioI. Control. 2002;16(2):145-148.