THE EFFECT OF MUTATION ON MICRO PROPAGATION OF Crocus sativus L.

Main Article Content

Z. SARGAZI MOGHADDAM
N. MOSHTAGHI
A. SHARIFI
A. BAGHERI

Abstract

Saffron is an important medicinal plant widely cultivated for nutrition and economic properties. The mutation can be used to increase the variation in this plant. The aim of this study was to evaluate of the effect of gamma radiation on the in vitro cormogenesis and the corm size and then acclimatization of the corms. Three ecotypes of the saffron corms were collected from Mashhad, Torbat-e Heidarieh and Sarayan provinces in Iran. Gamma radiation with the intensities of 2.5, 5, 7.5 and 10 Gray were used for corm treatment. Then, for In vitro propagation, the SH medium containing 3 mg/L BAP and 1 mg/L NAA was applied. The highest survival rate was observed in the 2.5 Gray treatment of apical buds, in Torbat-e Heydarieh ecotype with 90% survival. After two months, the bud number on each explant was determined. The highest bud number was observed in 2.5 Gray dose in the Sarayan ecotype with 11.03 buds per explant. The buds were transferred to a room with the temperature of 15°C and a photoperiod of 10/14 hrs (light/dark). The Rooting was done on a SH medium containing 2 mg/L BAP with 9% sucrose. The best treatment for cormogenesis induction was 1 mg/L IBA in ½ SH medium. Then, the MS medium with 6% sucrose was used to increase the size of corms in the dark condition at 25°C which led to a mean diameter increase of 1.22 cm. Finally, the corms were transferred to cocopit-perlite pots to check the survival rate. The acclimatization percentage was 91.66%. This study showed that the lower dose of Gamma ray can increase the number of sprouts and thereby the number of corm production.

Keywords:
In vitro, irradiation, propagation, saffron, corm.

Article Details

How to Cite
MOGHADDAM, Z. S., MOSHTAGHI, N., SHARIFI, A., & BAGHERI, A. (2019). THE EFFECT OF MUTATION ON MICRO PROPAGATION OF Crocus sativus L. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 20(23-24), 1059–1065. Retrieved from http://www.ikprress.org/index.php/PCBMB/article/view/4812
Section
Original Research Article

References

Agayev Y, Shakib A, Soheilivand S, Fathi M. Breeding of Saffron (Crocus sativus): Possibilities and problems. Paper Presented at the II International Symposium on Saffron Biology and Technology. 2006; 739.

Mir J, Ahmed N, Singh D, Khan M, Zaffer S, Shafi W. Breeding and biotechnological opportunities in saffron crop improvement. African Journal of Agricultural Research. 2015;10(9):970-974.

Devi K, Sharma M, Singh M, Singh Ahuja P. In vitro cormlet production and growth evaluation under greenhouse conditions in Saffron (Crocus sativus L.)– A commercially important crop. Engineering in Life Sciences. 2011;11(2):189-194.

Khan MA, Nagoo S, Naseer S, Nehvi F, Zargar SM. Induced mutation as a tool for improving corm multiplication in Saffron (Crocus sativus L.). Journal of Phytology. 2011;3(7):8-10.

Rastegari J, Vedadi S, Ghaffari M. Induce genetic variation by gamma-ray on buds of saffron corm. Journal of Nuclear Science and Technology. 2007;40:46-41.

Jahandar F, Iranloo A, Qaderi M, Rahimi M. Determination of proper dosage of gamma rays to induce genetic mutations in Saffron (Crocus sativus L.). The First National Conference on New Opportunities for Agricultural Production and Employment in the East of Country, Birjand University; 2017.

Lagram K, El Caid MB, El Aaouam S, Lachheb M, El Mousadik A, Serghini MA. In vitro shoot regeneration and development of microcorms of Moroccan Saffron (Crocus sativus L.). Atlas Journal of Plant Biology. 2016;50-55.

Ding B, Bai S, Wu Y, Fan X. Induction of callus and regeneration of plantlets from corm of Crocus sativus L. Chih wu Hsueh pao= Acta Botanica Sinica; 1981.

Gantait S, Vahedi M. In vitro regeneration of high value spice Crocus sativus L.: A concise appraisal. Journal of Applied Research on Medicinal and Aromatic Plants. 2015;2(4):124-133.

Quadri RR, Kamili AN, Husaini AM, Shah AM, Teixeira da Silva J. In vitro studies on cormogenesis and maximization of corm size in Saffron. Journal of Functional Plant Science and Biotechnology. 2010;4:132-135.

Schenk RU, Hildebrandt AC. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany. 1972;50(1):199-204.

Nehvi F, Wani S, Dar S, Makhdoomi M, Allie B, Mir Z. Biological interventions for enhancing Saffron productivity in Kashmir. Acta Horticulturae. 2007;739:25-29.

Salwee Y, Nehvi F. Creation of genetic variability in Saffron (Crocus sativus) using induced mutation. Journal of Cell and Tissue Research. 2014;14(2):32-37.

Kashtwari M, Wani AA, Dhar MK, Jan S, Kamili AN. Development of an efficient In vitro mutagenesis protocol for genetic improvement of Saffron (Crocus sativus L.). Physiology and Molecular Biology of Plants. 2018;24(5):951-962.

Nehvi F, Khan M, Lone AA, Maqhdoomi M, Wani SA, Yousuf V, Yasmin S. Effect of radiation and chemical mutagen on variability in Saffron (Crocus sativus L.). III International Symposium on Saffron: Forthcoming Challenges in Cultivation, Research and Economics. 2009;850:67-74.

Yasmin S, Nehvi F. Phenological growth stages of Saffron (Crocus sativus L.) under temperate conditions of Jammu and Kashmir-India. Int. J. Curr. Microbiol. App. Sci. 2018;7(4):3797-3814.

Bagri J, Yadav A, Anwar K, Dkhar J, Singla-Pareek SL, Pareek A. Metabolic shift in sugars and amino acids regulates sprouting in Saffron corm. Scientific Reports. 2017;7(1):11904.

Menia M, Iqbal S, Zahida R, Tahir S, Kanth R, Saad A, Hussian A. Production technology of saffron for enhancing productivity. Journal of Pharmacognosy and Phytochemistry. 2018;7(1):1033-1039.

Zeybek E, Önde S, Kaya Z. Improved In vitro micropropagation method with adventitious corms and roots for endangered Saffron. Central European Journal of Biology. 2012;7(1):138-145.