In vitro SHOOT INDUCTION IN ENDANGERED MEDICINAL PLANT Glycyrrhiza glabra L. USING SHOOT TIP EXPLANTS

Main Article Content

RUPESH MANEKAR
DNYANOBA JADHAV

Abstract

In the present research work, shoot tip explants were treated to establish an effective protocol of in vitro shoot induction. Full strength MS medium was examined with the various plant growth regulators like BAP and KIN alone and BAP with the combination of NAA, IBA, and IAA. Concentrations 1.0, 2.0, 3.0, 4.0 and 5.0 mgL-1 of BAP and KIN used alone and BAP (3.0 mgL-1) concentration combined with NAA, IBA, and IAA (1.0-2.0 mgL-1) were used for insertion of shoot tip explants. Amongst all, 3.0 mgL-1 concentration of BAP alone and BAP (3.0 mgL-1) combined with NAA (1.0 mgL-1) concentration were shown the most favorable results of all shoot parameters such as minimum days to induce shoot with the maximum percentage of shoot cultures and also showed the optimum shoot growth. Glycyrrhiza glabra L. is an important medicinal plant in which beneficial chemical component glycyrrhizin is present. The demand for this species increasing day to day as it contains precious pharmaceutical properties. Today, its habitat facing a problem of over-harvesting, so it is classified under the group of threatened species. Hence the current research was aimed to develop an in vitro shoot induction protocol to conserve the vital therapeutic plant Glycyrrhiza glabra L.

Keywords:
Cultures, explants, habitat, in vitro, pharmaceutical, protocol, therapeutic, threatened.

Article Details

How to Cite
MANEKAR, R., & JADHAV, D. (2019). In vitro SHOOT INDUCTION IN ENDANGERED MEDICINAL PLANT Glycyrrhiza glabra L. USING SHOOT TIP EXPLANTS. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 20(19-20), 890-895. Retrieved from http://www.ikprress.org/index.php/PCBMB/article/view/4762
Section
Original Research Article

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