Main Article Content
In the present research work, shoot tip explants were treated to establish an effective protocol of in vitro shoot induction. Full strength MS medium was examined with the various plant growth regulators like BAP and KIN alone and BAP with the combination of NAA, IBA, and IAA. Concentrations 1.0, 2.0, 3.0, 4.0 and 5.0 mgL-1 of BAP and KIN used alone and BAP (3.0 mgL-1) concentration combined with NAA, IBA, and IAA (1.0-2.0 mgL-1) were used for insertion of shoot tip explants. Amongst all, 3.0 mgL-1 concentration of BAP alone and BAP (3.0 mgL-1) combined with NAA (1.0 mgL-1) concentration were shown the most favorable results of all shoot parameters such as minimum days to induce shoot with the maximum percentage of shoot cultures and also showed the optimum shoot growth. Glycyrrhiza glabra L. is an important medicinal plant in which beneficial chemical component glycyrrhizin is present. The demand for this species increasing day to day as it contains precious pharmaceutical properties. Today, its habitat facing a problem of over-harvesting, so it is classified under the group of threatened species. Hence the current research was aimed to develop an in vitro shoot induction protocol to conserve the vital therapeutic plant Glycyrrhiza glabra L.
Sanaa, Hajera, Sabita Rani A. Micropropagation of Spilanthes acmella Murr. From nodal segment and Apical shoot tip cultures. Indian J. Sci. Res. 2017;13(1):92-98.
Sawaengsak W, Saisavoey T, Chuntaratin P, Karnchanatat A. Micropropagation of the medicinal herb Glycyrrhizin glabra L., through shoot tip explant culture and glycyrrhizin detection. Int. Res. J. Plant Sci. 2011;2:129-136.
He JX, Akao T, Nishino T, Tan T. The influence of commonly prescribed synthetic drugs for peptide ulcer on the pharmacokinetic fate of glycyrrhizin from Shaoyao-Gancao-tang. Biol. Pharm. Bull. 2001;24:1395-1399.
Mousami Debnath. Clonal propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. Journal of Medicinal Plants Research. 2008;2:045-051.
Matsui S, Matsumoto H, Sonoda Y, Ando K, Aizu-Yokota E, Sato T, Kasahara, T. Glycyrrhizin and related compounds down-regulate the production of inflammatory chemokines IL-8 and eotaxin 1 in a human lung fibroblast cell line. Int. Immuno-pharmacol. 2004;4:1633-1644.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473-497.
Biswas, Kakoli, Biswas, Ashima, Rajesh. In-vitro Clonal Propagation of an Indian Medicinal Plant- Justicia procumbens. International Journal of Applied Agri-cultural Research. 2017;12(2):239-245.
Asl MN, Hosseinzadeh H. Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytother. Res. 2008;22:709-724.
Duke JA. Handbook of legumes of world economic importance. Plenum Press, New York. 1981;90-92.
Henzab AL, Maha T, Elbadawi, Amira O, Ibrahim, Fatima H, Sidky, Rehab. Establishment of an efficient in vitro regeneration protocol for mass propagation via organogenesis of endangered plant Rhanterium epapposum in Qatar. Global Advanced Research Journal of Agricultural Science. 2018;7(4):25-129.
Gibson MR. Glycyrrhiza in old and new perspectives. Lloydia. 1978;41:348-354.
Hong YK, Wu HT, Ma T, Liu WJ, He XJ. Effects of Glycyrrhiza glabra polysaccharides on immune and antioxidant activities in high-fat mice. Int. J. Biol. Macromol. 2009;45(1):61-64.
Thorne Research. Inc. Glycyrrhiza glabra Monograph, Alternative Medicine Review. 2005;10(3):230-237.
Zhang, Yanni, Dongling Sun, Shangchun Hu. In vitro propagation of medicinal and ornamental plant Lysimachia davurica. Eur. J. Hortic. Sci. 2017;82(1):54-60.
Manisegaran I, Muthu G, Mannangatti E, Govindhan KU, Sethu T, Sethuraman R. An efficient micropropagation of Achyranthes aspera L. using shoot tip explants. Int. J. Curr. Res. Biosci. Plant Biol. 2018;5(2):76-84.
Bianchetti, Ricardo Ernesto, Cristiano Ferrara de Resende, Vinícius Sacramento Pacheco, Felipe Floriano Dornellas, Aline Mystica Silva de Oliveira, Jober Condé Evangelista Freitas, Paulo Henrique Pereira Peixoto. An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L. African Journal of Biotechnology. 2017;16(9):418-428.
Deb CR, Rout GR, Mao AA, Nandi SK, Singh RK, Vijayan D, Langhu T, Kikon ZP, Pradhan S, Tariq Mohd, Swain D. In vitro propagation of some threatened plant species of India. Current Science. 2018;114(3):567-575.
Sharma V, Boonen J, Chauhan NS, Thakur M, De Spiegeleer B, Dixit VK. Spilanthes acmella ethanolic flower extract: LC-MS alkyl amide profiling and its effects on sexual behavior in male rats. Phyto-medicine. 2011;18(13):1161-69.
Shin YW, Bae EA, Lee B, Lee SH, Kim JA, Kim YS, Kim DH. In vitro and in vivo antiallergic effects of Glycyrrhiza glabra and its components. Planta Med. 2007;73(3):257-61.
Wang ZY, Nixon DW. Licorice and cancer. Nutr. Cancer. 2001;39:1-11.
Olukoga A, Donaldson D. Historical perspective on health. The history of liquorice: The plant, its extracts, cultivation, commercialization and etymology. J. Roy Soc. Health. 1998; 118(5):300-304.
Rani G, Talwar D, Nagpal A, Virk, GS. Micropropagation of Coleus blumei from nodal segments and shoot tips. Biologia Plantarum. 2006;50(4):496- 500.
Reshi NA, Sudarshana MS, Girish HV. In vitro micropropagation of Anisochilus carnosus (L). Wall. Journal of Applied Pharmaceutical Science. 2017;7(07):098-102.
Gupta V, Kak A, Singh BB. Studies of germination and seedling vigor in liquorice (Glycyrrhiza glabra). Journal of medicinal and Aromatic plant Sciences. 1997;2:412-413.
Kaur, Preetpal, Sharma, Anju, Soni, Vineet. Micropropagation and induction of in vitro flowering in Tridax procumbens L. Biotechnol Res. 2017;3(4):85-88.
Mohan SN, Jayakumar KS, Hemanthakumar AS, Preetha TS. An efficient microcloning of Spilanthes ciliata Kunth. For Conservation and Sustainable Utilization. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS). 2018; 13(1-IV):10-17.