SOIL PHYSICOCHEMICAL PROPERTIES AND DIVERSITY OF ARBUSCULAR MYCORRHIZAL FUNGI IN THE RHIZOSPHERE OF SUGARCANE IN THE GHARB REGION OF MOROCCO

Main Article Content

ECH-CHEDDADI SARA
OUALLAL IMANE
MOUHSSINE FATINE
OUHADDACH MOUSSA
EL YACOUBI HOUDA
EL GOUMI YOUNES
ROCHDI ATMANE

Abstract

A study was conducted to investigate the soil physicochemical properties, the diversity of endogenous AMF associated with sugarcane, and to assess possible correlations among the soil physicochemical factors and the fungal community structure. The physicochemical analysis of the soils showed that they are moderately calcareous, calcareous, salty, not very salty to unsalted, with slightly alkaline hydrogen potentials. Concerning the soils fertility, they are moderately rich in organic matter and has medium to high levels of mineral nitrogen, assimilable phosphorus, and exchangeable potassium. The roots of the sugarcane were mycorrhized and showed endomycorrhizal structures of the AMF type. The intensity of mycorrhizal colonization and soil mycorrhizal potential varied from site to site. However, root colonization expressed as mycorrhization frequency was always high and varied significantly between the six sites (94 to 100%). The average density of AMF spores is 206.16 spores/100 g of soil. By analyzing this specific richness in AMF in rhizosphere soils of sugarcane, seven morphotypes were isolated. Analysis of the morpho-anatomical characteristics of isolated spores of the sugarcane rhizosphere revealed that these morphotypes belonged to three genera (Glomus, Acaulospora and Scutellospora). However, the genus Glomus has shown a large abundance. Variance analysis showed a very highly significant (P <.0001) difference in the number of spores between sites and between morphotypes. The morphotype Septoglomus constrictum and Funneliformis mosseae showed the major proportion of spores relative to other morphotypes. Principal component analysis (PCA) reveals an apparent correlation between the abundance of AMF morphotypes and edaphic factors.

Keywords:
Saccharum officinarum, rhizosphere, mycorrhizas, physicochemical parameters.

Article Details

How to Cite
SARA, E.-C., IMANE, O., FATINE, M., MOUSSA, O., HOUDA, E. Y., YOUNES, E. G., & ATMANE, R. (2019). SOIL PHYSICOCHEMICAL PROPERTIES AND DIVERSITY OF ARBUSCULAR MYCORRHIZAL FUNGI IN THE RHIZOSPHERE OF SUGARCANE IN THE GHARB REGION OF MOROCCO. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 20(19-20), 828–845. Retrieved from http://www.ikprress.org/index.php/PCBMB/article/view/4753
Section
Original Research Article

References

Medeiros CD, Neto JRF, Oliveira MT, Rivas R, Pandolfi V, Kido ÉA, Baldani JI, Santos MG. Photosynthesis, antioxidant activities and transcriptional responses in two sugarcane cultivars under salt stress. Acta Physiol Plant. 2014;36(2):447–59.

Redani L. Compétitivité, valorisation des ressources et objectifs de sécurité alimentaire pour la filière sucrière au Maroc. Université de liège Gembloux agro-bio Tech. 2015;60.

Driouech F. Distribution des précipitations hivernales sur le Maroc dans le cadre d’un changement climatique: Descente d’échelle et incertitudes. Université de toulouse, CNRM/Météo-France. 2010;89.

Simões WL, Calgaro M, Coelho SD, Batista D, Moisés DS, Souza A. Growth of sugar cane varieties under salinity. Rev. Ceres. 2016;63:2.

Duponnois R, Bâ AM, Galiana A, Baudoin E, Sanguin H, Lebrun M, Prin Y. Biotechnologie et mycorrhyzation contrôlée en milieu tropical. Des champignons symbiotiques contre la désertification: Ecosystèmes méditerranéens, tropicaux et insulaires. Marseille. 2013;262-279.

Asmelash F, Bekele T, Birhane E. The potential role of Arbuscular mycorrhizal fungi in the restoration of degraded lands. Front. Microbiol. 2016;7:1095.

Ekpélikpézé OS, Agre P, Dossou-Aminon I, Adjatin A, Dassou A, Dansi1 A. Characterization of sugarcane (Saccharum officinarum L.) cultivars of republic of Benin. Int. J. Curr. Res. Biosci. Plant Biol. 2016;3(5):147-156.

Xihui X, Chen C, Zhou Z, Zehua S, Yahua C, Jiandong J, Zhenguo S. The influence of environmental factors on communities of arbuscular mycorrhizal fungi associated with Chenopodium ambrosioides revealed by MiSeq sequencing investigation. Scientific Reports. 2016;7:45134.

Office régional de la mise en valeur agricole de la région du Gharb (20172018).

Petard J. Notes techniques laboratoire commun d'analyses, les méthodes d'analyse. Tome 1 Analyses de sols. ORSTOM, Nouméa; 1993.

Anne P. Sur le dosage rapide du carbone organique dans les sols. Agron. 1945;2:161-172.

Olsen SR, Cole CV, Watanabe FS, Dean LA. Estimation of available phosphorus in soils by with NaHCO3; 1954.

Rodier J. L’analyse de l’eau: Eaux Naturelles, eaux résiduaires, eau de mer 7ème édition. Paris: Dunod. 1984;1365.

Baize D. Guides des analyses courantes en pedologie. Paris: INRA. 1988;172.

Trouvelot A, Kough JL, Gianinazzi V. (). Mesure de taux de mycorhization VA d'un système radiculaire. Recherche de méthodes d'estimation ayant une signification fonctionnelle. In physiological and genetic aspects of mycorhizical, V. Gianinazzi-Pearson et S. Gianinazzi). INRA, Paris. 1986;217-221.

Philips JM, Hayman DS. Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc. 1970;55:158-161.

Lachapelle J. Microscopie, methodes d’examen, colorations. Revue du Cercle de Mycologie. 2004;4:35-71.

Gerdeman JW, Nicolson TH. Spore of mycorrhizal endogone species extracted from soil by wet sieving and decanting. Trans. Brit. Mycol. Soc. 1963;46:235-244.

Morton JB, Benny GL. Revised classification of arbuscular mycorrhizal fungi (Zygomycetes): A new order, Glomales, two new suborders, Glominae and Gigasporinae and two new families, Acaulosporaceae, with an emendation of Glomaceae. Mycotaxon. 1990;37:471–491.

Brundrett M, Bougher N, Dell B, Grove T, Malajczuk N. Working with mycorrhizas in forestry and agriculture. ACIAR Monograph. 1996;32.

International Culture Collection of (Vesicular) Arbuscular Mycorrhizae.
Available:http://invam.caf.wvu.edu

Sieverding E. Vesicular-arbuscular mycorrhiza management in tropical agrosystems. Deutsche (GTZ). Germany. 1991;371.

Sanon AA. D.E.A. National de science du sol: Rôle des champignons mycorhiziens à arbuscules dans les mécanismes régissant la co-existence entre espèces végétales. France. 2005;4-6.

Ward JH. Hierarchical grouping to optimize an objective function. Journal of the American Statistical Association. 1963;58:236-244.

Durand JH. Les sols irrigables. Etude pédologique. Presses Universitaire de France. Agence de Coopération Culturelle et Technique. 1983;338.

Ayers RS, Westcot DW. Water quality for agriculture. FAO Irrigation and Drainage. Rev. 1., FAO, Rome; 1985.

Roose E. Restauration de la productivité des sols tropicaux et méditerranéens Contribution à l'agroécologie. Institut de recherche pour le développement Montpellier. 2015;13.

Grieken M, Lynam T, Coggan A, Whitten S, Kroon F. Cost effectiveness of design-based water quality improvement regulations in the Great Barrier Reef Catchments Agriculture, Ecosystems & Environment. 2013;180:157-165.

Fall CS, Dia D, Msangi S. La politique des biocarburants au Sénégal: Évaluation de l’intégration potentielle du bioéthanol issu de la canne à sucre. Bureau d’Analyses Macro-Économiques de l’Institut Sénégalais de Recherches Agricoles, DT 2; 2012.

Cartmill AD, Valdez-Aguilar LA, Bryan DL, Alarcón A. Arbuscular mycorrhizal fungi enhance tolerance of vinca to high alkalinity in irrigation water. Scientia Horticulturae. 2008;115:275-284.

Meena VS, Maurya BR, Verma JP, Aeron A, Kumar A, Kim K, Bajpai VK. Potassium solubilizing rhizobacteria: Isolation, identification and K-release dynamics from waste mica. Ecol. Eng. 2015;81:340–347.

Eibisch N, Schroll R, Fub R, Mikutta R, Helfrich M, Flessa H. Pyrochars and hydrochars differently alter the sorption of the herbicide isoproturon in an agricultural soil. Chemosphere. 2015;119: 155–162.

Grieve CM, Grattan SR, Maas EV. Plantes tolérance au sel. En WW. Wallender, KK. Tanji (eds.) Manuel ASCE et Reports on Engineering Practice. Évaluation et gestion de la salinité agricole (2e édition). ASCE, Reston, VA. Chapitre. 2012;13:405-459.

Hinsinger P, Betencourt E, Bernard L, Brauman A, Plassard C, Shen JB, Tang XY, Zhang FS. P for two sharing a scarce resource: Soil phosphorus acquisition in the rhizosphere of intercropped species. Plant Physiology. 2011;156:1078-1086.

Suresh N, Nelson R. Diversity of arbuscular mycorrhizal fungi in the rhizosphere of sugarcane. European Journal of Experimental. Biology. 2015;53:13-19.

Artib M, Chliyeh M, Touati J, Talbi Z, Selmaoui K, Ouazzani Touhami A, Benkirane R, Douira A. Study of arbuscular mycorrhizal fungi diversity in the rhizosphere of citrus grown in Morocco. IJAPBC. 2016;5(3):2277.

Cui X, Hu J, Wang J, Yang JS, Lin XG. Reclamation negatively influences arbuscular mycorrhizal fungal community structure and diversity in coastal saline-alkaline land in Eastern China as revealed by Illumina sequencing. Appl. Soil Ecol. 2016;98:140–149.

Gehring CA. Mycorrhizal mediation of soil. 2017;501-509.

Gong MG, Ming T, Zhang QM, Feng XX. Effects of climatic and edaphic factors on arbuscular mycorrhizal fungi in the rhizosphere of Hippophae rhamnoides in the Loess Plateau, China. Acta Ecol. Sin. 2012;32:62–67.

Ouallal I, Abbas Y, Ech-cheddadi S, Ouajdi M, Ouhadach M, El Yacoubi H, Benaissa K, El Goumi Y, Rochdi A. Diversité des champignons endomycorhiziens de l’arganier et potentiel mycorhizogène des sols rhizosphériques des arganeraies du Sud-Ouest marocain. Bois et Forêts des Tropiques. 2018;338:73-86.

McCary MA, Zellner M, Wise DH. The role of plant–mycorrhizal mutualisms in deterring plant invasions: Insights from an individual‐based model. Ecology and Evolution. 2018;9:2018–2030.

Ferrol N, Calvente R, Cano C, Barea JM, Azcón-Aguilar C. Analysing arbuscular mycorrhizal fungal diversity in shrub-associated resource islands from a desertification threatened semiarid Mediterranean ecosystem. Applied Soil Ecology. 2003;26:11-19.

Abbas Y. Microorganismes de la rhizosphère des Tétraclinaies: Un outil pour optimiser la régénération assistée du Tetraclinis articulata Vahl. Master. Thèse de Doctorat National, Université Mohamed V, Rabat – Maroc; 2014.

Ndoye WF, Kane A, Ngonkeu Mangaptch E, Bakhoum N, Sanon A, Diouf D, Oureye Sy M, Baudoin E, Noba K, Prin Y. Changes in land use system and environmental factors affect arbuscular mycorrhizal fungal density and diversity and enzyme activities in rhizospheric soils of Acacia senegal (L.). ISRN Ecology. 2012;13.

Piotrowski JS, Denich T, Klironomos JN, Graham JM, Rillig MC. New Phytol. 2004;164:365–373.

Parihar M, Rakshit A, Singh HB, Rana K. Diversity of arbuscular mycorrhizal fungi in alkaline soils of hot sub humid eco-region of Middle Gangetic Plains of India. Acta Agriculturae Scandinavica, Section B, Soil and Plant Science. 2019;1651-1913.

Jinping W, Geo GW, Zhang B, Zhongming Y, Zhiyuan F, Yingdan Y, Lingjun Z, Shilin M, Jinchi Z. Arbuscular mycorrhizal fungi associated with tree species in a planted forest of Eastern China. Forests. 2019;10:424.

Ogura-Tsujita Y, Sakoda A, Ebihara A. Arbuscular mycorrhiza formation in cordate gametophytes of two ferns, Angiopteris lygodiifolia and Osmunda japonica. Plant Res. 2013;41:50.

Zahraeni K, Yunus M, Nur A, Laode A, Ifayanti R. Exploration of arbuscular mycorrhizal fungi from sugarcane rhizosphere in south Sulawesi. International Journal of Scientific Technology Research. 2014;3(1):2277-8616.

Datta P, Kulkarni M. Arbuscular mycorrhizal fungal diversity in sugarcane rhizosphere in relation with soil properties. Not. Sci. Biol. 2012;4(1):66-74.

Börstler B, Thiéry O, Sýkorová Z, Berner A, Redecker D. Diversity of mitochondrial large subunit r DNA haplotypes of Glomus intraradices in two agricultural field experiments and two semi‐natural grasslands. Molecular Ecology. 2010;19:1497–1511.

Hodge A. Chapter two - Interactions between arbuscular mycorrhizal fungi and organic material substrates. Advances in Applied Microbiology. 2014;89:47-99.

Moreira-Souza M, Trufem SFB, Gomes-da-Costa Mycorrhiza. 2003;13:211.

Rachid CTCC, Santos AL, Piccolo MC, Balieiro FC, Coutinho HLC, Peixoto RS, et al. Effect of sugarcane burning or green harvest methods on the Brazilian Cerrado soil bacterial community structure. Plos One. 2013;8(3):59342.

Meddich A, Hafidi M, Ait El mokhtar M, Boumezzough A. Caractérisation des paramètres physicochimiques et des potentialités mycorhizogènes des sols salés de la palmeraie Nord-est de Marrakech. Mater. Environ. Sci. 2015;6(9):2469-2475.

Sidhoum W. Diversité des mycorhizes arbusculaires chez la variété « Sigoise » d’olivier: Etude de leurs efficacités sur la croissance des plantes. République algérienne démocratique et populaire ministre de l’enseignement supérieur et de la recherche scientifique. 2011;4-51.

Saad. Etudes des endomycorhizes de la variété sigoise d’olivier et essai de leur application à des boutures semi-ligneuses multipliées sous nébulisation. Mémoire de Magister en Biotechnologie. Oran. 2009; 124.