EFFECT OF SILVER NITRATE IN COMBINATION WITH SOME PLANT GROWTH REGULATORS ON MICROPROPAGATION OF Lantana camara L. - A VALUABLE MEDICINAL PLANT

Main Article Content

NGUYEN QUANG DUC TIEN
TRAN THI THANH NHAN
LE THI ANH THU
NGUYEN HOANG LOC

Abstract

The present study investigates the effect of silver nitrate in combination with some plant growth regulators on shoot regeneration and rooting of Lantana camara with the aim of improving the micropropagation for this valuable medicinal plant species. Results show that Murashige and Skoog (MS) medium with 1.5 mg/L benzylaminopurine (BAP) and 1 mg/L silver nitrate, or 1 mg/L kinetin and 1 mg/L silver nitrate produced highest number of shoots/explant after 4 weeks of culture, 9.5 and 7.8 respectively. Single shoots formed roots on MS medium with indoleacetic acid (IAA) or indolebutyric acid (IBA) in combination with silver nitrate. Highest number of roots/shoots appeared on the medium supplemented with 1 mg/L IBA (or 1 mg/L IAA) and 1 mg/L silver nitrate, including 5.33 or 5.6 primary roots and 19.54 or 14.76 secondary roots. Plantlets grown healthy under in vitro culture condition.

Keywords:
Lantana camara, micropropagation, nodal segment, silver nitrate, top segment.

Article Details

How to Cite
TIEN, N. Q. D., NHAN, T. T. T., THU, L. T. A., & LOC, N. H. (2019). EFFECT OF SILVER NITRATE IN COMBINATION WITH SOME PLANT GROWTH REGULATORS ON MICROPROPAGATION OF Lantana camara L. - A VALUABLE MEDICINAL PLANT. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 20(15-16), 634–642. Retrieved from http://www.ikprress.org/index.php/PCBMB/article/view/4708
Section
Original Research Article

References

Sharma B, Kumar P. Bioefficacy of Lantana camara L. against some human pathogens. Indian J. Pharm. Sci. 2009; 71:589-593.

Goncalves E, Herrera I, Duarte M, Bustamante RO, Lampo M, Velásquez G, Sharma GP, García-Range S. Global invasion of Lantana camara: Has the climatic niche been conserved across continents? Plos One. 2014;9:e111468.

Samani EN, Jabbarzadeh Z, Ghobadi S, Motamedi M. Effect of different concentrations of plant growth regulators on micropropagation of Lantana camara. J. Med. Plants Res. 2014;8:1299-1303.

Kalita S, Kumar G, Karthik L, Rao KVB. A review on medicinal properties of Lantana camara Linn. Res. J. Pharm. Technol. 2012;5:711-715.

Reddy NM. Lantana camara Linn. Chemical constituents and medicinal properties: A review. Sch. Acad. J. Pharm. 2013;2:445-448.

Bhakta D, Ganjewala D. Effect of leaf positions on total phenolics, flavonoids and proantho-cyanidins content and antioxidant activities in Lantana camara (L). J. Sci. Res. 2009;1:363-369.

Venkatachalam T, Kumar VK, Selvi PK, Maske AO, Kumar NS. Physicochemical and preliminary phytochemical studies on the Lantana camara (L.) fruits. Int. J. Pharm. Pharm. Sci. 2011;3:52-54.

Mary KV. Studies on phytochemical screening and antibacterial activities of Lantana camara Linn. Plant Sci. Feed. 2011;1:74-79.

Kalita S, Kumar G, Karthik L, Rao KVB. Phytochemical composition and in vitro hemolytic activity of Lantana camara L. (Verbenaceae) leaves. Pharmacologyonline. 2011;1:59-67.

Veraplakorn V. Micropropagation and callus induction of Lantana camara L.-A medicinal plant. Agric. Nat. Resour. 2016;50:338-344.

Veraplakorn V. In vitro micropropagation and allelopathic effect of lantana (Lantana camara L.). Agric. Nat. Resour. 2017;51:478-484.

Abdellatef E, Khalafalla MM. Ethylene inhibitors promote in vitro regeneration of medium staple cotton (Gossypium hirsutum L.) cultivar Barac B-67. Adv. Nat. Appl. Sci. 2008;2:178-184.

Kumar V, Parvatam G, Ravishankar GA. AgNO3-a potential regulator of ethylene activity and plant growth modulator. Electron. J. Biotechnol. 2009;1.
DOI: 10.2225/vol12-issue2-fulltext-1

Osman MG, Khalafalla M. Promotion of in vitro shoot formation from shoot tip of tomato (Lycopersicon esculentum Mill. Cv. Omdurman) by ethylene inhibitors. Int. J. Curr. Res. 2010;4:82-86.

Sandra AT, Maira O. Effect of culture medium consistence and silver nitrate on micropropagation of two potato (Solanum tuberosum) cultivars. Rev. Colomb. Biotecnol. 2013;15:55-62.

Tamimi SM. Effects of ethylene inhibitors, silver nitrate (AgNO3), cobalt chloride (CoCl2) and aminooxyacetic acid (AOA), on in vitro shoot induction and rooting of banana (Musa acuminata L.). Afr. J. Biotechnol. 2015;14:2510-2516.

Tahoori F, Majd A, Nejadsattari T, Ofoghi H, Iranbakhsh A. Effects of silver nitrate (AgNO3) on growth and anatomical structure of vegetative organs of liquorice (Glycyrrhiza glabra L.) under in vitro condition. Plant Omics J. 2018;11:153-160.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962;15:473-497.

Giridhar P, Obul-Reddy B, Ravishankar GA. Silver nitrate influences in vitro shoot multiplication and root formation in Vanilla planifolia Andr. Curr. Sci. 2001;81:1166-1170.

Anantasaran J, Kanchanapoom K. Influence of medium formula and silver nitrate on in vitro plant regeneration of Zinnia cultivars. Songklanakarin J. Sci. Technol. 2008;30:1-6.

Ozudogru EA, Ozden-Tokatli Y, Akcin A. Effect of silver nitrate on multiple shoot formation of Virginia-type peanut through shoot tip culture. In Vitro Cell. Dev. Biol. – Plant. 2005;41:151-156.

Gutiérrez-Miceli FA, Arias L, Juarez-Rodriguez N, Abud-Archila M, Amaro-Reyes A, Dendooven L. Optimization of growth regulators and silver nitrate for micropropagation of Dianthus caryophyllus L. with the aid of a response surface experimental design. In vitro Cell. Dev. Biol. - Plant. 2010;46:57-63.

Chawla S, Bansal YK. Enhanced effect of additives on direct and adventitious shoot multiplication in Helicteres isora L. Int. J. Pharm. Sci. Rev. Res. 2014;27:261-265.