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Eranthis longistipitata Regel (Ranunculaceae) is an endemic plant of Central Asia and one of the most popular among decorative bulbs. There is a need for the development of fast and controlled propagation methods of this species. The purpose of this study is to identify the features of in vitro culture of E. longistipitata seeds and embryos. The effect of various pretreatment methods on germination of E. longistipitata seeds in in vitro culture was studied – cold stratification, treatment with gibberellic acid (GA3) and a combination of cold stratification and embryo culture treatment. It is shown that mature seeds of E. longistipitata exhibit an underdeveloped embryo, and for complete maturation of embryos in in vitro culture, it is necessary to incubate the seeds at +4°C in the dark for 2 months, and then at +7°C under a 16/8 photoperiod regime for 2 months. The optimal method of seed pretreatment is a combination of cold stratification and embryo culture, when the number of viable explants attained 23%. GA3 seed treatment did not foster embryo development and seed germination of E. longistipitata. The study has shown that a combination of cold stratification and embryo culture methods is necessary to overcome the dormancy of E. longistipitata seeds in vitro.
Vetchinkina EM, Shirnina IV, Shirnin S. Yu, Molkanova OI. The conservation of rare species of plants in the in vitro genetic collections. Bulletin of the Baltic Federal University Named after I. Kant. 2012;7:109-118.
Cammue BP, Peeters B, Peumans WJ. Isolation and partial characterization of an N-acetylgalactosamine-specific lectin from winter-aconite (Eranthis hyemalis) root tubers. Biochemical Journal. 1985;227(3): 949-955.
George O, Solscheid C, Bertolo E, Fell J, Lisgarten D. Extraction and purication of the lectin found in the tubers of Eranthis hyemalis (winter aconite). Journal of Integrated Omics. 2011;1(2):268-271.
Ziv M, Lilien-Kipnis H. Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant Cell Reports. 2000;19:845–850.
Özcan S, Parmaksız I, Mirici S, Cocu S, Uranbey S, Ipek A, Sancak C, Sarıhan EO, Gurbuz B, Sevimay CS, Arslan N. Efficient in vitro bulblet regeneration from immature embryos of endemic and endangered geophyte species in Sternbergia, Muscari and Fritillaria genera / Z. Xu, J. Li, Y. Xue, W. Yang, Eds. Biotechnology and Sustainable Agriculture 2006 and Beyond. Dordrecht, The Netherlands: Springer. 2007;381–383.
Podwyszyńska M. The mechanisms of in vitro storage organ formation in ornamental geophytes. Floriculture and ornamental biotechnology. Bulbous Ornamentals. 2012;6(1):9-23.
Gheisari M, Miri SM. In vitro callus induction and bulblet regeneration of Hyacinth (Hyacinthus orientalis L.). Plant Cell Biotechnology and Molecular Biology. 2017;18(3-4):145-155.
Padma S, Shivakameshwari MN. In vitro regeneration using bulb and leaf explants of Urginea indica Kunth. Plant Cell Biotechnology and Molecular Biology. 2017;18(7-8):464-472.
Muraseva DS, Novikova TI. Efficient protocol for in vitro propagation from bulb scale explants of Fritillaria ruthenica Wikstr. (Liliaceae), a rare ornamental species. Rendiconti Lincei. Scienze Fisiche e Naturali. 2018;29:491–497.
Wu Y, Ma YD, Li Y, Zhang L, Xia YP. Plantlet regeneration from primary callus cultures of Lilium brownii F.E.Br. ex Miellez var. giganteum G. Y. Li & Z. H. Chen, a rare bulbous germplasm. In vitro Cell. Dev. Biol.-Plant. 2019;55:44.
Shipchinsky NV. Genus 513. Vesennik - Eranthis Salisb. Flora of the USSR: in 30 tons / chap. Ed. V.L. Komarov. - M.; L.: Publishing House of the Academy of Sciences of the USSR,. - T. 7 / Ed. volumes B. K. Shishkin. 1937;60-62.
Haccius B. Histogenetische Untersuchungen an Wurzelhaube und Kotyledonarscheide geophiler Keimpflanzen (Podophyllum und Eranthis). Planta. 1953;41:439–458.
Frost‐Christensen H. Embryo development in ripe seeds of Eranthis hyemalis and its relation to gibberellic acid. Physiologia Plantarum. 1974;30:200-205.
Tipirdamaz R, Gömürgen AN. The effects of temperature and gibberellic acid on germination of Eranthis hyemalis (L.) Salisb. Seeds Turk J Bot. 2000;24:143– 145.
Bullowa S, Ozeri Y. Role of temperature and growth regulators in germination in Anemone coronaria L. Austr. J. Plant Physiol. 1975;2:91-100.
Forbis TA, Diggle PA. Subnivean embryo development in the alpine herb Caltha leptosepala (Ranunculaceae). Can. J. Bot. 2001;79:635-642.
Baskin JM, Baskin CC. A classification system for seed dormancy. Seed Sci. Res. 2004;14:1-16.
Nikolaeva MG, Lyanguzova IV, Pozdova LM. Seed biology. SPb. 1999;231.
Vetchinkina EM. Biological features of in vitro cultivation of seeds and embryos of rare plant species. Theses of the candidate of biological sciences. Moscow. 2010;22.
Lange T. Molecular biology of gibberellin synthesis. Planta. 1998;204:409-419.
Srivastava N, Sharma V, Dobriyal AK, Kamal B, Gupta S, Jadon VS. Influence of pre-sowing treatments on in vitro seed germination of Ativisha (Aconitum heterophyllum Wall) of Uttarakhand. Biotechnology. 2011;10:215-219.
Pozdova LM, Razumova MV. Dormancy of seeds. Embryology of flowering plants. Terminology and concepts. St. Petersburg: Peace and Family. 1997;823.
Zaprometov MN. Phenolic compounds: Distribution, metabolism and functions in plants. LVI Timiryazev Reading. M: Nauka. 1996;45.