INFLUENCE OF POLYAMINES ON In vitro REGENERATION AND ANTIBACTERIAL ACTIVITY IN Sesamum indicum (L.)

Main Article Content

DHANDAPANI ELAYARAJA
MARKANDAN MANICKAVASAGAM

Abstract

The present study was carried out to develop an efficient plant regeneration protocol for Sesamum indicum (L.) from cotyledon explants prepared from 10-day-old in vitro grown plantlets. Cotyledon explants inoculated on MS medium containing 2 mg l−1 N6–Benzyladenine (BA) and 30 mg l−1 spermidine induced the higher number of shoots (23.43 shoots/explant) and the induced shoots were elongated (5.63 cm/shoots) in the same medium. The maximum frequency (92.33%) of root induction was recorded in MS medium fortified with 10 mg l−1 putrescine. Up to 95% of the regenerated plantlets acclimatized and developed further under the greenhouse conditions. Antibacterial activity of the regenerated plant extracts exhibited resistance against various pathogens such as, Escherichia coli, Vibrio cholerae, Bacillus subtilis and Micrococcus luteus. Highest zone of inhibition (13 mm) was recorded for B. subtilis and M. luteus at 50 μg ml−1 extract.

Keywords:
Cotyledon, in vitro acclimatization, polyamines, sesame, shoot multiplication

Article Details

How to Cite
ELAYARAJA, D., & MANICKAVASAGAM, M. (2019). INFLUENCE OF POLYAMINES ON In vitro REGENERATION AND ANTIBACTERIAL ACTIVITY IN Sesamum indicum (L.). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 20(13-14), 568-576. Retrieved from http://www.ikprress.org/index.php/PCBMB/article/view/4677
Section
Original Research Article

References

Bedigian D, Seigler DS, Harlan JR. Sesamin, sesamolin and the origin of sesame. Biochem Syst Ecol. 1985;13(2):133-139.

Chattopadhyaya B, Banerjee J, Basu A, Sen SK, Mrinalmaiti K. Shoot induction and regeneration using internodal transverse thin cell layer culture in Sesamum indicum L. Plant Biotechnol Rep. 2010;4:173-178.

Gangopadhyay G, Poddar R, Gupta S. Micropropagation of sesame (Sesamum indicum L.) by in vitro multiple shoot production from nodal explants. Phytomorphology. 1998;48:83–90.

Taskin KM, Turgut K. In vitro regeneration of sesame (Sesamum indicum L.). Turk J Bot. 1997; 21:15–18.

Rao KR, Vaidyanath K. Callus induction and morphogenesis in sesame (Sesamum indicum L.). Adv Plant Sci. 1997a;10:21–26.

Were BA, Gudu S, Onkware AO, Carlsson A, Welander M. In vitro regeneration of sesame (Sesamum indicum L.) from seedling cotyledon and hypocotyl explants. Plant Cell Tiss Org Cult. 2006;85:235– 239.

Yadav M, Chaudhary D, Sainger M, Jaiwal PK. Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.). Plant Cell Tissue Organ Cult. 2010;103:377–386.

George L, Bapat VA, Rao PS. In vitro multiplication of sesame (Sesamum indicum) through tissue culture. Ann Bot. 1987;60:17–21.

Rao KR, Vaidyanath K. Induction of multiple shoots from seedling shoot tips of different varieties of Sesamum. Indian J Plant Physiol. 1997b;2:257–261.

Seo H, Park T, Kim Y, Kim H, Yun S, Park K, Oh M, Choi M, Paik C, Lee Y, Choi Y. High-frequency plant regeneration via adventitious shoot formation from de-embryonated cotyledon explants of Sesamum indicum L. In Vitro Cell Dev Biol Plant. 2007;43:209–214.

Sivanandhan G, Mariashibu TS, Arun M, Rajesh M, Kasthurirengan S, Selvaraj N, Ganapathi A. The effect of polyamines on the efficiency of multiplication and rooting of Withaniasomnifera (L.) Dunal and content of some with anolides in obtained plants. Acta Physiol Plant. 2011;33:2279–2288.

Arun M, Subramanyam K, Theboral J, Ganapathi A, Manickavasagam M. Optimized shoot regeneration for Indian soybean: the influence of exogenous polyamines. Plant Cell Tiss Organ Cult. 2014;117:305–309.

Vasudevan V, Subramanyam K, Elayaraja D, Karthik S, Vasudevan A, Manickavasagam M. Assessment of the efficacy of amino acids and polyamines on regeneration of watermelon (Citrullus lanatusThunb.) and analysis of genetic fidelity of regenerated plants by SCoT and RAPD markers. Plant Cell Tiss Organ Cult. 2017;130:681–687.

Galston AW. Polyamines as modulators of plant development. Bioscience. 1983;33:382–388.

Bais HP, Ravishankar GA. Role of polyamines in the ontogeny and their biotechnological applications. Plant Cell Tiss Org Cult. 2002;69:1–34.

Kakkar RK, Sawhney VK. Polyamine research in plants: a changing perspective. Physiol Plant. 2002;116:281–292.

Desai HV, Mehta AR. Changes in polyamine levels during shoot formation, root formation and callus induction in cultured Passiflora leaf discs. J. Plant. Physiol. 1985;119:45–53.

Chi GL, Lin WS, Lee JEE, Pua EC. Role of polyamines on de novo shoot morphogenesis from cotyledons of Brassica campestris spp. Pekinensis (Lour) Olsson in vitro. Plant Cell Rep. 1994;13:323–329.

Tian CE, Li RG, Guan H. Relationship between polyamines and morphogenesis in cotyledons of Cucumis melo L. cultured in vitro. Acta Bot. Sin. 1994;36:219–222.

Pua CE, Sim EG, Chi LG, Kong FL. Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyls explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey) in vitro. Plant Cell Rep. 1996;15:685–690.

Vasudevan A, Selvaraj N, Ganapathi A, Kasthurirengan S, Ramesh Anbazhagan V, Manickavasagam M, Choi C. Leucine and spermidine enhance shoot differentiation in cucumber (Cucumis sativus L.). In vitro Cell Dev Biol Plant. 2008;44(4):300–306.

Murarhige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 1962;15:473–497.

Sathishkumar G, Rajkuberan C, Manikandan K, Prabukumar S, Daniel John J, Sivaramakrishnan S. Facile biosynthesis of antimicrobial zinc oxide (ZnO) nanoflakes using leaf extract of Couroupita guianensis Aubl. Mater. Lett. 2017;383–386.

Raja A, Jayabalan N. In vitro shoot regeneration and flowering of sesame (Sesamum indicum L.) cv. J Agric Technol. 2011;7:1089–1096.

Chowdhury S, Basu A, Kundu S. A new high-frequency Agrobacterium-mediated transformation technique for Sesamum indicum L. using de-embryonated cotyledon as explant. Protoplasma. 2014;251:1175-90.

Bhattacharyya J, Chakraborty A, Mitra J, Chakraborty S, Pradhan S, Manna A, Sikdar N, Soumitra Kumar Sen. Genetic transformation of cultivated sesame (Sesamum indicum L.cv Rama) through particle bombardment using 5-day-old apical, meristematic tissues of germinating seedlings. Plant Cell Tiss Organ Cult. 2015;123:455-466.

Kumar V, Sharma A, Prasad BCN, Gururaj HB, Giridhar P, Ravishankar GA. Direct shoot bud induction and plant regeneration in Capsicum frutescens Mill.: influence of polyamines and polarity. Acta Physiol Plant. 2007;29(1):11.

Sivakumar S, Siva G, Sathish S, Prem Kumar G, Vigneshwaran S, Vinoth S, Senthil Kumar T, Sathishkumar R, Jayabalan N. Influence of exogenous polyamines and plant growth regulators on high frequency in vitro mass propagation of Gloriosa superba L. and its colchicine content. Biocatalysis and Agricultural Biotechnology. 2019;101030.

Thiruvengadam M, Chung LM, Chun SC. Influence of polyamines on in vitro organogenesis in bitter melon (Momordica charantia L.). Afr J Biotechnol. 2012;6(19):3579–3585.

Geneve RL, Hackett WP. Ethylene evolution and endogenous polyamine levels during adventitious root formation in English ivy. Curr Top Plant Physiol. 1990;5:332–334.

Thiruvengadam M, Chung IM. Phenolic compound production and biological activities from in vitro regenerated plants of gherkin (Cucumis anguria L.). Electron J Biotechnol. 2015;18:295–301.

Sathish D, Vasudevan V, Theboral J, Elayaraja D, Appunu C, Siva R, Manickavasagam M. Efficient direct plant regeneration from immature leaf roll explants of sugarcane (Saccharum officinarum L.) using polyamines and assessment of genetic fidelity by SCoT markers. In vitro Cellular & Developmental Biology –Plant. 2018;54(4):399-412.

Tarenghi E, Carre M, Martin-Tanguy J. Effects of inhibitors of polyamine biosynthesis and of polyamines on strawberry microcutting growth and development. Plant Cell Tiss Org Cult. 1995;42:47–55.

Nag S, Saha K, Chowdhuri M. Role of auxin and polyamine in adventitious root formation at the base of mung bean cuttings. Indian J. Plant Physiol. 1999;4:247–255.

Cushnie TPT, Lamb AJ. Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents. 2011;99–107.

Boobalan S, Kamalanathan D. Spermidine influences enhanced micropropagation and antibacterial activity in Aervajavanica (Burm. F.) Shult. Industrial Crops and Products. 2019;137:187-196.

Shikanga EA, Combrinck S, Regnier T. South African Lippia herbal infusions: Total phenolic content, antioxidant and antibacterial activities. South African Journal of Botany. 2010;567–571.