In vitro PROPAGATION OF Adenosma indianum (Lour.) Merr.

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TRUONG THI BICH PHUONG
PHAN THUY TRUC NHU
LAM THI NGOC THUY
TRAN THUY LAN
TRAN QUOC DUNG
HOANG TAN QUANG

Abstract

This paper presents the results of in vitro propagation of Adenosma indianum (Lour.) Merr. The obtained results indicated that shoot regeneration from stem explant achieved on MS basal medium supplemented with 0.5 mg/L BAP after 4 weeks of culture, 46.7% of samples regenerating shoots with an average value of 1.8 shoots per sample. The MS medium containing 0.30 mg/L NAA was suitable for callus induction from leaves and containing 0.50 mg/L BAP and 0.10 mg/L NAA had a highest ability to produce buds from callus, with a ratio of 43.52%, and 3.43 shoots/callus. The multiplication coefficient was greatest on MS medium consist of 0.5 mg/L BAP and 0.3 mg/L IBA, average number of shoots per explant was 10.03 after 6 weeks of culture. The MS medium supplemented with 0.25 mg/L NAA was optimal for rooting (average value of 7.17 roots per shoot). The plantlets were acclimatized and transplanted successfully in the coconut coir pots, the plantlet survival rate was 91.67% after two months of cultivation. The in vitro propagation procedure of A. indianum (Lour.) Merr was developed successfully, shoots can be regenerated directly from axillary bud or callus and in vitro plantlets grow well in greenhouse.
Keywords:
Adenosma indianum, callus induction, medicinal plant, shoot regeneration, shoot tip, stem

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How to Cite
BICH PHUONG, T. T., TRUC NHU, P. T., NGOC THUY, L. T., THUY LAN, T., QUOC DUNG, T., & TAN QUANG, H. (2019). In vitro PROPAGATION OF Adenosma indianum (Lour.) Merr. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 20(5-6), 222–231. Retrieved from http://www.ikprress.org/index.php/PCBMB/article/view/4601
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Original Research Article