SECONDARY METABOLITES PRODUCTION FROM CELL SUSPENSION CULTURE OF Citrus aurantium L.

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DORSAF KRIAA
M'BAREK BEN NACEUR
ANNA RITA MEREU
GRAZIA MARIA SCARPA

Abstract

Flavonoid contents in fruit and tissues of Citrus aurantium are related to antioxidant activity, and are of particular interest in the pharmaceutical industry. The aim of the present work was to develop a protocol for production of flavonoids, from Citrus aurantium cell suspensions. For this purpose, substrates containing different growth regulators, at different concentrations, were compared. The highest percentage of callus formation (100%), for cotyledons cultured in presence of mg.l-1 2,4-D and 2 mg.l-1 kinetin, and for ovules cultured in presence of 0.2 mg.l-1 2,4-D and 2 mg.l-1 kinetin was observed. The hormonal combination that gave the higher amount of fresh weight was 1 mg.l-1 2, 4D with 2 mg.l-1 kinetin for cotyledons and MS supplemented with 1 mg.l-1 or 0.2 mg.l-1 2,4 D and 2 mg.l-1 kinetin. The highest amount of total polyphenols and total flavonoids was obtained from cell suspensions starting from callus of ovules.

Keywords:
Flavonoid, bitter orange, in vitro culture, callus culture, suspension culture

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How to Cite
KRIAA, D., NACEUR, M., MEREU, A., & SCARPA, G. (2019). SECONDARY METABOLITES PRODUCTION FROM CELL SUSPENSION CULTURE OF Citrus aurantium L. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 20(3-4), 140-151. Retrieved from http://www.ikprress.org/index.php/PCBMB/article/view/4576
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Original Research Article