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The green fabrication of metal nanoparticles by using botanical extracts is significantly important for increasing research attention in nanotechnology, since it does not require high energy inputs or the production of highly toxic chemicalbyproducts. Here, silver (Ag), gold (Au) and their bimetallic (Ag/Au) nanoparticles (NPs) were green synthesized using the Crescentiaalata aqueous flower extract. Metal NPs were studied by spectroscopic (UV–visible spectroscopy, fluorescence spectroscopy, FT-IR spectroscopy, XRD and EDX) and microscopic (AFM and TEM) analysis. AFM and TEM showed that Ag and Au NPs had triangular and spherical morphologies, with an average size of 20 nm. Bimetallic Ag/Au NPs showed spherical shapes with an average size of 10 nm. Ag and Ag/Au bimetallic NPs showed high antibacterial and antibiofilm activities towards Gram-positive and Gram-negative bacteria. Overall, the proposed synthesis route of Ag, Au and Ag/Au bimetallic NPs can be exploited by the pharmaceutical industry to develop drugs effective in the fight against microbic infections.
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