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The Standardization absolutely of radioactive sources 153Sm to calibrate the nuclear medicine equipment had been carried out in PTKMR-BATAN. This is necessary because the radioactive sources used in the field of nuclear medicine has a very short half-life in other that to obtain a quality measurement results require special treatment. Besides that, the use of nuclear medicine techniques in Indonesia develop rapidly. All the radioactive sources were prepared by gravimetric methods using KERN ABT 220-5DM type balance, traceable to SI. Standardization of 153Sm has been carried out 4phi beta (LS) – gamma coincidence methods, while the impurities has been carried out by gamma spectrometry methods which calibrated using 133Ba, 152Eu, and 241Am. The result of absolutely measurement was 88.48 kBq/g with 1.36 % of the expanded uncertainty, in covered factor, k = 2. This value to be used for calibrating a Capintec CRC-7BT radionuclide calibrator which is a secondary standard instrument in PTKMR - BATAN. The results show that calibration factor for Capintec CRC-7BT dose calibrator is 1.05 by about 4.6 % of the expanded uncertainties.